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2010, 27(6):356-359.doi:

Genotyping Study on Some Strains of Legionella Pneumophila Serotype 1 in Shanghai by Pulsed-field Gel Electrophoresis


1. Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China

Received: 2009-10-11;  Published: 2017-06-26

Corresponding Author: ZHANG Xi , Email: xzhang@scdc.sh.cn  

[Objective] To study the genotypic characteristics of some Legionella pneumophila serotype 1 (Lp1) isolated from cooling tower water in public settings of Shanghai in 2007.

[Methods] Twenty-six environmental isolates, which were isolated from the cooling tower water, were identified as Lp1. The pulsed-field gel electrophoresis (PFGE)was applied to draw the fingerprintings of the isolates, while the cluster analysis was employed by BioNumerics software.

[Results] Fifteen distinguishable PFGE patterns were differentiated among 26 Lp1 strains. Most of the strains differed in genetic similarity, from 30.00% to 100.00%, with no predominant PFGE pattern. The majority of PFGE patterns were composed of the isolates from the same place, while most of the strains from same source were indentified as same PFGE pattern.

[Conclusion] Great genotypic diversity was found in Lp1 isolated from cooling tower water in public settings of Shanghai in 2007.

Key Words: legionella pneumophila serotype 1;  pulsed-field gel electrophoresis;  fingerprinting;  PFGE pattern 

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