[Objective] To investigate the adverse effects on F1 rats' spleen while the pregnant mother rats were exposed to nonylphenol(NP).
[Methods] Pregnant rats were exposed to NP via gavage at doses of 0, 50, 100 and 200 mg/kg by body weights respectively per day, from pregnant days 9 to 15. The level of malondialdehyde (MDA)and activity of super oxide dimutese(SOD) in F1 rats' spleen homogenate were detected respectively at the age of 60th day. The ultrastructure of spleen tissues were observed under electronic microscope.
[Results] Compared with the control group, the absolute and relative weight of spleen were decreased dramatically in the 200 mg/kg NP treated group and negative relation between the weight and dose was found (rspleen=-0.618, rcoefficient=-0.530, P<0.05). The SOD activity of spleen tissues decreased in NP 100 and 200 mg/kg exposed fetal rats, while the levels of MDA increased in NP 200 mg/kg exposed fetal rats. Both of them were in a dose-dependent manner(rSOD=-0.460, rMDA=0.747, P<0.05). The differences mentioned above were statistical significant compared to control group (P<0.05). The changes of the ultrastructrue were found among the spleen of NP 200 mg/kg group that characterized with chromatin condesed, clumped in circa-nuclei and mitochondrial tumefaction and vacuolization.
[Conclusion] NP can get across the placenta barrier to make the F1 offspring rats' spleen damage of lipid peroxidation.