FENG Le , LIN Yue , LI Shan , XU Ying , YANG Bing-yi , WANG Mei . Direct Determination of Lead in Mouse Blood by Graphite Furnace Atomic Absorption Spectrometry Optimized by Response Surface Methodology[J]. Journal of Environmental and Occupational Medicine, 2014, 31(7): 557-560. DOI: 10.13213/j.cnki.jeom.2014.0132
Citation: FENG Le , LIN Yue , LI Shan , XU Ying , YANG Bing-yi , WANG Mei . Direct Determination of Lead in Mouse Blood by Graphite Furnace Atomic Absorption Spectrometry Optimized by Response Surface Methodology[J]. Journal of Environmental and Occupational Medicine, 2014, 31(7): 557-560. DOI: 10.13213/j.cnki.jeom.2014.0132

Direct Determination of Lead in Mouse Blood by Graphite Furnace Atomic Absorption Spectrometry Optimized by Response Surface Methodology

  • Objective To explore a method for direct determination of lead in mouse blood by graphite furnace atomic absorption spectrometry (GFAAS) with optimization using response surface methodology.

    Conclusion After blood samples were diluted with 0.5% Triton X-100 solution, lead in blood was directly determined by GFAAS using a mixture solution of 0.05% PdCl2 and 0.1% HNO3 as modifier. The effects of matrix modifier dosage, ashing temperature, and atomization temperature on absorbance were examined by central composite design and response surface methodology.

    Results The optimum extraction conditions were as follows: ashing temperature, 760.85℃ ; atomization temperature, 2 486.80℃ ; and dosage of matrix modifier, 0.97 mL. Under the optimal conditions, the value of detection limit for Pb was 0.204 μg/L, and the relative standard deviation (RSD) for six replicate determinations of Pb was 1.76%. Lead in blood of mice was successfully detected at a concentration of 82.1 μg/L, and the recoveries were 96.5%-98.4%.

    Conclusion The method is simple, rapid, and accurate by direct injection without digestion or centrifugation, which can be applied to lead determination in mouse blood.

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