石惠惠, 柳晓琳, 李丹, 王永伟, 王强, 熊燕. 锆对小鼠肾脏的损伤作用[J]. 环境与职业医学, 2014, 31(1): 48-51. DOI: 10.13213/j.cnki.jeom.2014.0013
引用本文: 石惠惠, 柳晓琳, 李丹, 王永伟, 王强, 熊燕. 锆对小鼠肾脏的损伤作用[J]. 环境与职业医学, 2014, 31(1): 48-51. DOI: 10.13213/j.cnki.jeom.2014.0013
SHI Hui-hui , LIU Xiao-lin , LI Dan , WANG Yong-wei , WANG Qiang , XIONG Yan . Kidney Damage Induced by Zirconium in Mice[J]. Journal of Environmental and Occupational Medicine, 2014, 31(1): 48-51. DOI: 10.13213/j.cnki.jeom.2014.0013
Citation: SHI Hui-hui , LIU Xiao-lin , LI Dan , WANG Yong-wei , WANG Qiang , XIONG Yan . Kidney Damage Induced by Zirconium in Mice[J]. Journal of Environmental and Occupational Medicine, 2014, 31(1): 48-51. DOI: 10.13213/j.cnki.jeom.2014.0013

锆对小鼠肾脏的损伤作用

Kidney Damage Induced by Zirconium in Mice

  • 摘要: 目的 研究锆对小鼠肾脏的损伤。

    方法 将50只健康6~8周龄清洁级雄性昆明小鼠按体质量随机分成5组,分别为对照(生理盐水)组和0.855、1.710、3.420、17.100 mg/kg氧氯化锆染毒组,每组10只。采用腹腔注射方式进行染毒,染毒剂量为20 mL/kg,每日1次,连续染毒30 d。测定血清肌酐(Scr)和尿素氮(BUN)的含量及肾脏匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性和丙二醛(MDA)的含量。

    结果 与对照组相比,仅17.100 mg/kg氧氯化锆染毒组小鼠血清中Scr含量明显高于对照组,差异有统计学意义(P<0.05)。随着染毒剂量的升高,小鼠血清中Scr的含量呈上升趋势(r=0.609, P<0.001)。此外,与对照组比较, 1.710、3.420、17.100 mg/kg氧氯化锆染毒组小鼠肾脏匀浆中GSH-Px、SOD活性均下降, 17.100 mg/kg氧氯化锆染毒组小鼠肾脏匀浆中MDA的含量升高,差异有统计学意义(P<0.05)。随着氧氯化锆染毒剂量的升高,小鼠肾脏匀浆中的MDA含量呈上升趋势(r=0.755, P<0.001),SOD、GSH-Px活性均呈下降趋势(r=-0.570, P<0.001; r=-0.536, P=0.001)。0.855、1.710、3.420 mg/kg氧氯化锆染毒组小鼠肾脏肾小管上皮细胞轻度肿胀,有轻微炎细胞浸润,其中3.420 mg/kg染毒组小鼠肾小管上皮细胞部分损伤脱落;17.100 mg/kg染毒组小鼠肾脏肾小管管腔狭窄,多数上皮细胞损伤脱落。

    结论 在本实验范围内,低剂量的氧氯化锆对小鼠肾脏有轻微损伤,而高剂量(>17.100 mg/kg)的氧氯化锆对小鼠肾脏有明显的损伤作用。

     

    Abstract: Objective To observe the kidney damage induced by zirconium in mice.

    Methods A total of 50 male Kunming mice were randomly divided into five groups by body weight, and each group contained 10 mice. The five groups of mice were exposed by intraperitoneal injection with 0.000, 0.855, 1.710, 3.420, 17.100 mg/kg of zirconyl chloride solution. The exposure lasted for 30 consecutive days with a single dose of 20 mL/kg per day. After the exposure was finished, the contents of serum creatinine (Scr) and urea nitrogen in serum as well as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in the kidney sample homogenates were tested.

    Results Compared with the control group, only the 17.100 mg/kg group's Scr showed statistically significant difference (P<0.05). The content of Scr increased with the zirconyl chloride dose increasing (r=0.609, P<0.001). In the groups of 1.710, 3.420, 17.100 mg/kg, the SOD and GSH-Px activities in kidney homogenates reduced, and the MDA content increased in the group of 17.100 mg/kg (all P<0.05). The content of MDA increased while the activities of SOD and GSH-Px reduced along with the exposure dose increasing (r=0.755, P<0.001; r=-0.570, P<0.001; r=-0.536, P=0.001). The observation of selected kidney tissue samples found mildly swelling renal tubular epithelial cells and in flammatory cells infiltration in the groups of 0.855, 1.710, and 3.420 mg/kg. Partially damaged and detachment of the renal tubular epithelial cells were observed in the group of 3.420 mg/kg. The renal tubules were narrow and most epithelial cells were damaged in the group of 17.100 mg/kg.

    Conclusion The mice kidneys are slightly damaged by low doses of zirconyl chloride, and significant damages are found in high dose group (>17.100 mg/kg).

     

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