钟艳艳, 钟儒刚, 王明连, 刘洋, 马雪梅, 侯庆霞, 陈薛钗. 900 MHz手机射频辐射对体外细胞DNA损伤及EB病毒早期抗原的激活作用[J]. 环境与职业医学, 2014, 31(3): 180-185. DOI: 10.13213/j.cnki.jeom.2014.0044
引用本文: 钟艳艳, 钟儒刚, 王明连, 刘洋, 马雪梅, 侯庆霞, 陈薛钗. 900 MHz手机射频辐射对体外细胞DNA损伤及EB病毒早期抗原的激活作用[J]. 环境与职业医学, 2014, 31(3): 180-185. DOI: 10.13213/j.cnki.jeom.2014.0044
ZHONG Yan-yan , ZHONG Ru-gang , WANG Ming-lian , LIU Yang , MA Xue-mei , HOU Qing-xia , CHEN Xue-chai . DNA Damage in Vitro and Activation of Epstein-Barr Virus Early Antigen Induced by 900 MHz Mobile Phone Radiofrequency Radiation[J]. Journal of Environmental and Occupational Medicine, 2014, 31(3): 180-185. DOI: 10.13213/j.cnki.jeom.2014.0044
Citation: ZHONG Yan-yan , ZHONG Ru-gang , WANG Ming-lian , LIU Yang , MA Xue-mei , HOU Qing-xia , CHEN Xue-chai . DNA Damage in Vitro and Activation of Epstein-Barr Virus Early Antigen Induced by 900 MHz Mobile Phone Radiofrequency Radiation[J]. Journal of Environmental and Occupational Medicine, 2014, 31(3): 180-185. DOI: 10.13213/j.cnki.jeom.2014.0044

900 MHz手机射频辐射对体外细胞DNA损伤及EB病毒早期抗原的激活作用

DNA Damage in Vitro and Activation of Epstein-Barr Virus Early Antigen Induced by 900 MHz Mobile Phone Radiofrequency Radiation

  • 摘要: 目的 研究900 MHz、最大功率密度为10.644μW/cm2的手机射频辐射对体外细胞DNA损伤和病毒癌基因EB病毒早期抗原(EBV-EA)的激活作用。

    方法 (1)将NIH/3T3细胞分别暴露在手机辐射下0、1、2、4、6、8、12 h,并分别设定相应的正常对照组,用彗星电泳检测细胞DNA受损程度。(2)将Raji细胞分为A辐照(-) TPA (-)、B辐照(-) TPA (+)、C辐照(+) TPA (-)、D辐照(+) TPA (+)4组,接受手机辐照,强度为4 h/d,实验持续4周;每周结束时,B和D两组细胞分别使用促癌剂12-0-十四烷酰佛波醇-13-乙酸酯(TPA)1 ng/mL处理48 h。用免疫细胞化学检测各组细胞中EBV-EA的阳性表达情况。

    结果 当NIH/3T3细胞辐照2 h后,与对照组相比细胞Olive尾距有明显差异(P < 0.05),但在随后的2~12 h内的各个检测时间点上,Olive尾距并没有随着时间的增加而增加(P > 0.05);Raji细胞在实验4周后,辐照C、D组细胞的EBV-EA阳性表达率较A组明显增加(P < 0.05),并且在加入促癌剂TPA的D组,阳性表达率更加明显(P <0.01)。

    结论 900 MHz手机射频辐射会对细胞DNA产生一定的损伤,并且能够激活EBV-EA的表达。

     

    Abstract: Objective To probe the DNA damage and activation of Epstein-Barr virus early antigen (EBV-EA) induced by 900 MHz mobile phone radiofrequency radiation at an maximum power density of 10.644 µW/cm.

    Methods NIH/3T3 cells were exposed to mobile phone radiation for 0,1,2,4,6,8,12h,respectively and followed by DNA damage detection via comet assay;corresponding control groups were also set.Raji cells were divided into four groups:A,radiation (-) TPA (12-0-tetradecanoylphorbol-13-acetate)(-);B,radiation (-) TPA (+);C,radiation (+) TPA (-);D,radiation (+) TPA (+).Group C and D were exposed to mobile phone radiation,four hours per day,for four weeks.At the end of each week,group B and D were treated with 1 ng/mL TPA for forty-eight hours.Immunocytochemistry method was used to detect the activation of EBV-EA.

    Results The Olive tail moment of NIH/3T3 cells were significantly increased after exposed to the designed mobile phone radiation over two hours (P < 0.05).However,there were no significant increases in the other time points (P > 0.05).Moreover,after being exposed for four weeks,the positive rates of EBV-EA in group C and D were higher than that in group A (P < 0.05),and especially in the TPA treated group D showing the highest positive rate (P < 0.01).

    Conclusion Mobile phone radiofrequency radiation could cause damage to cellular DNA with the designed intensity and exposure protocol,and may activate the expression of EBV-EA.

     

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