杨章萍, 金铨, 周标, 刘少颖, 丁钢强, 朱益民. 性早熟女童血清中环境内分泌干扰物水平研究[J]. 环境与职业医学, 2014, 31(5): 331-335. DOI: 10.13213/j.cnki.jeom.2014.0076
引用本文: 杨章萍, 金铨, 周标, 刘少颖, 丁钢强, 朱益民. 性早熟女童血清中环境内分泌干扰物水平研究[J]. 环境与职业医学, 2014, 31(5): 331-335. DOI: 10.13213/j.cnki.jeom.2014.0076
YANG Zhang-ping , JIN Quan , ZHOU Biao , LIU Shao-ying , DING Gang-qiang , ZHU Yi-min . Environmental Endocrine Disruptors in Serum of Sexually Precocious Girls[J]. Journal of Environmental and Occupational Medicine, 2014, 31(5): 331-335. DOI: 10.13213/j.cnki.jeom.2014.0076
Citation: YANG Zhang-ping , JIN Quan , ZHOU Biao , LIU Shao-ying , DING Gang-qiang , ZHU Yi-min . Environmental Endocrine Disruptors in Serum of Sexually Precocious Girls[J]. Journal of Environmental and Occupational Medicine, 2014, 31(5): 331-335. DOI: 10.13213/j.cnki.jeom.2014.0076

性早熟女童血清中环境内分泌干扰物水平研究

Environmental Endocrine Disruptors in Serum of Sexually Precocious Girls

  • 摘要: 目的 了解女童血清中环境内分泌干扰物(EEDs)的水平及其与性早熟的关系。

    方法 采用病例对照研究,以2011年杭州市某儿童医院内分泌科确诊为性早熟的女童219人为病例组,杭州市4所小学的健康女童271人为对照组,采用超高效液质联用技术测定女童血清中有机氯农药DDT的代谢产物DDE和20种邻苯二甲酸酯(DEHP、DBP、DIDP、DIBP、DNOP、DHXP、DEP、DAP等),及其4种代谢产物(MEHP、MBP、MEP等),以及2种洗涤剂的降解产物(4-OP、4-NP)和双酚A,共28种EEDs的含量。采用多元线性回归方法分析检出的EEDs与女童性早熟的相关性。

    结果 病例组和对照组血清中检出14种EEDs,其中两组DDE、DEHP、DIBP、DBP、DIDP、MBP检出率均较高(50%~100%)。病例组DNOP(χ2=30.191, P<0.001)和DIDP(χ2=71.100, P<0.001)检出率显著高于对照组。病例组血清中DDE、DEHP、DNOP含量明显高于对照组(Z值分别为8.350、3.435、5.487, P<0.001);而DEP、DAP、DIBP、DBP、DHXP、MEHP、MBP、MEP含量则明显低于对照组(Z值分别为4.389、9.290、5.592、2.962、4.703、5.384、3.607、3.650, P<0.01);多元回归显示, DNOP(b=0.128, P<0.001)和DDE(b=0.357, P<0.001)的含量与性早熟呈正相关,而DAP(b=-0.183, P<0.001)和MEP(b=-0.055, P<0.01)的含量与性早熟呈负相关。

    结论 两组女童血清都检出EEDs, DDE、DNOP的水平升高可能与女童性早熟的发生相关, DEP、DAP、DIBP、DBP、DHXP、MEHP、MBP、MEP存在低检出水平。

     

    Abstract: Objective To examine the relationship of environmental endocrine disruptors (EEDs) in serum with precocious puberty in girls.

    Methods A case-control study was conducted among 219 girls with precocious puberty diagnosed by endocrinologists in a children's hospital (case group) and 271 healthy girls of four primary schools (control group) in Hangzhou in 2011. The concentrations of 28 EEDs, including 20 phthalates di(2-ethyl hexyl)phthalate (DEHP), di-n-butyl phthalate (DBP), diisodecyl ortho-phthalate (DIDP), di(isobutyl)-1, 2-benzenedicarboxylate (DIBP), di-n-octyl phthalate (DNOP), di-n-hexyl phthalate (DHXP), diethyl phthalate (DEP), dibutyl phthalate A (DAP), etc., 4 metabolites monoethylhexyl phthalate (MEHP), monobutyl phthalate (MBP), monoethyl phthalate (MEP), etc., 1, 1-dichloro-2, 2-bis(4-chlorophenyl)ethane (DDE), 4-octylphenol (4-OP), 4-nonylphenol (4-NP), and bisphenol (BPA), were detected in girls' serum by high performance liquid chromatography-mass spectrometry. Multiple linear regression models were used to analyze the correlation between the concentrations of EEDs and precocious puberty in girls.

    Results Totally 14 EEDs were detected, and DDE, DEHP, DIBP, DBP, DIDP, MBP presented high detection rates in both groups (50%-100%). The detection rates of DNOP (χ2=30.191, P<0.001) and DIDP (χ2=71.100, P<0.001) were significantly higher in the case group than in the control group. The serum contents of DDE, DEHP, and DNOP in the case group were much higher than those in the control group (Z=8.350, 3.435, and 5.487, respectively, P<0.001). The serum contents of DEP, DAP, DIBP, DBP, DHXP, MEHP, MBP, and MEP were obviously lower in case group than in the control group (Z=4.389, 9.290, 5.592, 2.962, 4.703, 5.384, 3.607, and 3.650, respectively, P<0.01). Multiple linear regression models showed that concentrations of DNOP (b=0.128, P<0.001) and DDE (b=0.357, P<0.001) were positively associated with precocious puberty, while DAP (b=-0.183, P<0.001) and MEP (b=-0.055, P<0.01) were negatively associated.

    Conclusion EEDs positive are detected in both girl groups. The elevated serum levels of DDE and DNOP may be associated with precocious puberty in girls. The le vels of DEP, DAP, DIBP, DBP, DHXP, MEHP, MBP, and MEP are low in girls with precocious puberty.

     

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