陈玉之, 李冰燕, 孙静, 陈丽莉, 韦晔, 童建. 线粒体部分敲除对氡染毒人支气管上皮细胞损伤效应的影响[J]. 环境与职业医学, 2014, 31(6): 438-441. DOI: 10.13213/j.cnki.jeom.2014.0101
引用本文: 陈玉之, 李冰燕, 孙静, 陈丽莉, 韦晔, 童建. 线粒体部分敲除对氡染毒人支气管上皮细胞损伤效应的影响[J]. 环境与职业医学, 2014, 31(6): 438-441. DOI: 10.13213/j.cnki.jeom.2014.0101
CHEN Yu-zhi , LI Bing-yan , SUN Jing , CHEN Li-li , WEI Ye , TONG Jian . Effects of Mitochondrial DNA Partial Knockout on Injury of Human Bronchial Epithelial Cells Induced by Radon Exposure[J]. Journal of Environmental and Occupational Medicine, 2014, 31(6): 438-441. DOI: 10.13213/j.cnki.jeom.2014.0101
Citation: CHEN Yu-zhi , LI Bing-yan , SUN Jing , CHEN Li-li , WEI Ye , TONG Jian . Effects of Mitochondrial DNA Partial Knockout on Injury of Human Bronchial Epithelial Cells Induced by Radon Exposure[J]. Journal of Environmental and Occupational Medicine, 2014, 31(6): 438-441. DOI: 10.13213/j.cnki.jeom.2014.0101

线粒体部分敲除对氡染毒人支气管上皮细胞损伤效应的影响

Effects of Mitochondrial DNA Partial Knockout on Injury of Human Bronchial Epithelial Cells Induced by Radon Exposure

  • 摘要: 目的 观察线粒体部分敲除对氡染毒人支气管上皮细胞损伤效应的影响。

    方法 分别对线粒体DNA部分敲除的人支气管上皮细胞(ρ-HBE细胞)和未经敲除的ρ+HBE细胞暴露20 000 Bq/m3的氡及氡子体,观察两种受试细胞第10代(低剂量组, ρ+HBE-Rn10和ρ-HBE-Rn10)及第30代(高剂量组, ρ+HBE-Rn30和ρ-HBE-Rn30)细胞的增殖能力、细胞周期以及活性氧(ROS)水平的变化。

    结果 氡染毒后,线粒体部分敲除后高、低剂量组(ρ-HBE-Rn30, ρ-HBE-Rn10)的细胞克隆形成率(PE)和细胞相对存活分数(SF)均高于敲除前(P<0.05);敲除前高剂量组(ρ+HBE-Rn30)的PE和SF均低于低剂量组(ρ+HBE-Rn10) (P<0.05),敲除后则高于低剂量组(P<0.05)。低剂量组ρ+HBE细胞中的S期细胞减少,G2/M期细胞增加,高剂量组的S期细胞增加, G1期细胞减少;各染毒剂量的ρ+HBE细胞和ρ-HBE细胞, ROS水平均升高(P<0.05)。

    结论 经氡染毒后, ρ-HBE细胞在生长速度、细胞周期、ROS产生量等生物学指标上与ρ+HBE细胞有明显改变,表明氡所产生的细胞损伤效应与线粒体的结构和功能障碍密切相关。

     

    Abstract: Objective To observe the impact of mitochondrial DNA partial knockout on the injury of human bronchial epithelial cells exposed to radon gas.

    Methods Bronchial epithelial cells with (ρ-HBE cells) or without (ρ+HBE cells) mitochondrial DNA partial knockout were exposed to 20 000 Bq/m3 radon and radon daughters to observe the changes in cell proliferation, cell cycle, and intracellular reactive oxygen species (ROS) levels of cells at 10 passages (low-dose groups:ρ+HBE-Rn10 and ρ-HBE-Rn10) and 30 passages (high-dose groups:ρ+HBE-Rn30 and ρ-HBE-Rn30).

    Results After radon exposure, the plating efficiency (PE) and the survival fraction (SF) of the ρ-HBE-Rn10 and ρ-HBE-Rn30 cells were higher than those of the ρ+HBE cells (P<0.05). PE and SF were lower in the ρ+HBE-Rn30 cells than the ρ+HBE-Rn10 cells (P<0.05), while higher in the ρ-HBE-Rn30 cells than the ρ-HBE-Rn10 cells (P<0.05). S phase decreased and G2/M phase of the ρ+HBE-Rn10 cells increased, while the ρ+HBE-Rn30 cells showed the opposite effects. The ROS levels were increased in all exposure groups.

    Conclusion Compared to the ρ+HBE cells, the ρ-HBE cells have a significant change in cell proliferation, cell cycle, and intracellular ROS generation after radon exposure, indicating that the radon induced cell injury is associated with mitochondrial structure and impairment.

     

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