不同产地的温石棉对V79细胞的毒性对比研究
Contrastive Study on Toxicities of Chrysotiles with Different Origins on V79 Cells
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摘要:
目的 评价我国青海茫崖、甘肃阿克塞、陕西陕南和四川新康的温石棉对V79细胞的毒性。 方法 采集我国四大矿区的4种温石棉,红外光谱分析其表面主要活性基团, X光荧光衍射分析仪分析主要化学成分。4种粉尘与V79细胞相互作用,比较4种石棉细胞存活率,测定培养液乳酸脱氢酶(lactate dehydrogenase, LDH)活力、葡萄糖浓度及钙(Ca)、镁(Mg)、铁(Fe)、铝(Al)、硅(Si)元素含量。 结果 除四川新康温石棉无外羟基,其余3种温石棉的表面主要活性基团基本一致,化学组成主要是二氧化硅(SiO2)及氧化镁(MgO),但含量不同。细胞存活率以陕南温石棉最高,茫崖温石棉最低, 4种温石棉的LDH、葡萄糖及Mg含量比较,差异有统计学意义(P<0.05, P<0.01),仅茫崖与阿克塞矿区温石棉Si含量差异有统计学意义(P<0.01); 4种温石棉Ca、Mg、Fe、Al含量差异均无统计学意义。 结论 不同矿床所产的温石棉,因生成条件不尽相同,表现活性基团不同,则细胞的毒性亦不同。 Abstract:Objective To evaluate the toxicities on V79 cells of chrysotiles from four major mining areas in China. Methods Four kinds of chrysotiles were collected from China's four major mining areas and analyzed for surface activity groups by infrared spectroscopy, and main chemical components with X-ray fluorescence diffract analyzer. V79 cells were administered with the four kinds of chrysotile dust and followed by detections of cell viability, lactic dehydrogenase (LDH) activity, glucose (GLU) concentration, as well as Ca, Mg, Fe, Al, and Si contents. Results The surface activity groups of the three kinds of chrysotiles were in substantial agreement except the Xinkang chrysotiles which showed no outer hydroxyl groups, and the main chemical components were SiO2 and MgO with varied contents. The cell viability of the Shaannan chrysotiles was the highest and the Mangya chrysotiles the lowest. The four kinds of chrysotiles had distinct differences in LDH, GLU, and Mg (P<0.05, P<0.01); Si was only showed different between the Mangya and the Akesai groups (P<0.01); no differences in Ca, Mg, Fe, Al were found in all four kinds of chrysotiles. Conclusion Chrysotiles with different origins show different toxicities because of their different forming conditions and activity groups.
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