石墨炉原子吸收光谱法测定尿中铝的方法研究
Determination of Aluminum in Urine by Graphite Furnace Atomic Absorption Spectrometry
-
摘要:
目的 建立石墨炉原子吸收光谱测定尿中铝的方法。 方法 应用0.10%曲拉通-100(TritonX-100)和0.50%硝酸直接稀释样品,石墨炉原子吸收光谱法直接测定尿中铝的含量。 结果 通过试验,该方法的线性相关系数为0.999,检出限为1.02 μg/L,回收率为91.8%~104.9%,相对标准偏差在3.9%~7.9%,尿样在4℃冰箱中至少可以保存14 d。 结论 所建立的分析方法灵敏、简便、准确,可测定尿中铝。 Abstract:Objective To establish a method for determination of aluminum in urine by graphite furnace atomic absorption spectrometry (GFAAS). Methods Samples were diluted with 0.10% TritonX-100 and 0.50% HNO. Concentrations of urinary aluminum were detected by GFAAS. Results The correlation coefficient was 0.999, the limit of detection was 1.02 μg/L, the recovery rate ranged from 91.8% to 104.9%, with the relative standard deviation of 3.9%-7.9%. The urine samples could be stored at 4℃ for at least 14 days. Conclusion The method is sensitive, convenient, and accurate, and thus can be used to determine aluminum in urine.