固相萃取-气相色谱法测定血中氯丁二烯的方法研究

Determination of Chloroprene in Blood by Solid Phase Extraction Gas Chromatography

摘要: 目的建立血样中氯丁二烯的固相萃取-气相色谱测定方法。

方法血样中的氯丁二烯经C18 小柱富集,用甲醇洗脱,洗脱液经带有ECD检测器的气相色谱仪检测,外标法定量。

结果该方法的线性范围为0.42~20.0 μg/L,相关系数(r)=0.999 8,方法检出限为0.42 μg/L;血样中最低检出质量浓度0.42 μg/L;样品加标回收率为82.0%~91.3%。

结论该方法灵敏度高,操作方便,选择性好,适用于职业性氯丁二烯中毒的临床诊断和治疗过程。

Abstract: Objective To establish a method for the determination of chloroprene in blood by solid phase extraction gas chromatography.

Methods Chloroprene in blood samples was concentrated by C18 column and eluted with methanol. The elution was determined by gas chromatography with ECD detector and quantified by external standard method.

Results The linear range of the proposed method was 0.42-20.0 μg/L, with a correlation coefficient (r) of 0.999 8. The detection limit was 0.42 μg/L. The minimum detectable concentration in the blood samples was 0.42 μg/L. The spiked recovery rate was 82.0%-91.3%.

Conclusion The method is of high sensitivity, easy operation, and high specificity. It can be used for clinical diagnosis and treatment of occupational chloroprene poisoning.