纳米硒与亚硒酸钠在高碘致小鼠氧化损伤中的干预作用
Intervention Effects of Nano Selenium and Sodium Selenite on Oxidative Damage Induced by Excess Iodine Intake in Mice
-
摘要:
目的 研究比较纳米硒和亚硒酸钠对高碘致小鼠部分组织氧化损伤的干预作用。 方法 将48只雄性昆明小鼠被随机分为适碘组50 μg/L碘酸钾(KIO3)、高碘组(3 000 μg/L KIO3)、亚硒酸钠组(3 000 μg/L KIO3+0.193 mg/kg硒)、纳米硒组(3 000 μg/L KIO3+0.193 mg/kg硒),每组12只。4周后处死小鼠,测定血清、肝脏、肾脏组织的谷胱甘肽过氧化物酶(GSH-Px)活力、以及丙二醛(MDA)水平。观察甲状腺病理变化。 结果 与适碘组相比,高碘组血清、肝脏和肾脏中GSH-Px活力均明显下降,差异有统计学意义(P<0.05);肝脏中MDA含量下降,差异有统计学意义(P<0.05);肝脏和肾脏脏器系数均增加,差异有统计学意义(P<0.05)。与高碘组相比,亚硒酸钠组血清和肝脏中GSH-Px活力均明显升高,差异有统计学意义(P<0.05);肾脏中MDA含量增加,差异有统计学意义(P<0.05);肝脏脏器系数下降,差异有统计学意义(P<0.05);血清和肝脏中MDA含量均下降。纳米硒组血清、肝脏和肾脏中GSH-Px活力均明显增加,差异有统计学意义(P<0.05);血清和肝脏中MDA含量均明显下降,在肾脏中增加,差异有统计学意义(P<0.05);肾脏脏器系数下降,差异有统计学意义(P<0.05)。高碘组甲状腺组织出现弥漫胶质性甲状腺肿。 结论 高碘摄入可致小鼠氧化性损伤,补硒有一定干预作用,纳米硒的干预效果优于亚硒酸钠。 Abstract:Objective To compare the intervention effects of nano selenium and sodium selenite on oxidative damage in selected mouse tissues induced by high iodine. Methods Forty-eight male Kunming mice were divided into four groups:normal io dine group (50 µg/L KIO3), high iodine group (3 000 µgL KIO3), sodium selenite group (3 000 µg/L KIO3 plus 0.193 mg/kg Se), nano selenium group (3 000 µg/L KIO3 plus 0.193 mg/kg Se). Four weeks later, glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in serum, liver, and kidney were determined. Pathological changes of thyroid were also observed. Results Compared with the normal iodine group, in the high iodine group, the GSH-Px activities in serum, liver, and kidney were reduced (P<0.05); the MDA content in liver was also lowered (P<0.05); the tissue organ coefficients of liver and kidney were elevated (P<0.05). Compared with the high iodine group, in the sodium selenite group, the GSH-Px activities in liver and serum were increased (P<0.05); the MDA content in kidney was increased (P<0.05); the organ coefficient of liver tissue was decreased (P<0.05). In the nano selenium group, compared with the high iodine group, the GSH-Px activities in liver, kidney, and serum were increased (P<0.05); the MDA contents decreased significantly in liver and serum, but increased significantly in kidney (P<0.05); the organ coefficient of kidney tissue decreased significantly (P<0.05). The mice in the high iodine group showed colloid goiter. Conclusion High iodine in take may result in oxidative damage in mice, and selenium supplementation could exert favorable effects on it. Intervention effect of nano selenium is better than that of sodium selenite.
下载:
