宋鑫, 王芹, 杭学宇, 王媛. 超高效液相色谱-串联质谱法检测动物源性食品中的4种β-受体激动剂[J]. 环境与职业医学, 2016, 33(2): 193-196. DOI: 10.13213/j.cnki.jeom.2016.15333
引用本文: 宋鑫, 王芹, 杭学宇, 王媛. 超高效液相色谱-串联质谱法检测动物源性食品中的4种β-受体激动剂[J]. 环境与职业医学, 2016, 33(2): 193-196. DOI: 10.13213/j.cnki.jeom.2016.15333
SONG Xin, WANG Qin, HANG Xue-yu, WANG Yuan. Determination of Four β-Agonists in Animal Derived Food by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Journal of Environmental and Occupational Medicine, 2016, 33(2): 193-196. DOI: 10.13213/j.cnki.jeom.2016.15333
Citation: SONG Xin, WANG Qin, HANG Xue-yu, WANG Yuan. Determination of Four β-Agonists in Animal Derived Food by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Journal of Environmental and Occupational Medicine, 2016, 33(2): 193-196. DOI: 10.13213/j.cnki.jeom.2016.15333

超高效液相色谱-串联质谱法检测动物源性食品中的4种β-受体激动剂

Determination of Four β-Agonists in Animal Derived Food by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

  • 摘要: 目的

    建立超高效液相色谱-串联质谱法(UPLC-MS/MS)定量测定肉类中的克伦特罗、莱克多巴胺、沙丁胺醇及特布他林4种β-受体激动剂的方法。

    方法

    样品经β-葡萄糖醛苷酶/芳基硫酸酯酶酶解,再经SLW固相萃取柱净化后,采用UPLC-MS/MS方法测定4种β-受体激动剂。

    结果

    4种β-受体激动剂在0.1~20 μg/L测定的范围内线性关系良好(r>0.999 6),方法的检出限为0.03~0.10 μg/kg(S/N=3)。在加标水平为2、5、10 μg/kg时,空白样品的加标回收率为70.3%~97.1%,相对标准偏差为0.25%~4.50%。

    结论

    该方法分析时间短、选择性好、灵敏度高,适用于肉类中的β-受体激动剂的检测。

     

    Abstract: Objective

    To establish a method for the determination of four β-agonists including clenbuterol, ractopamine, salbutamol, and terbutaline in meat products induced by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).

    Methods

    The test samples were enzymatically hydrolyzed with β-glucuronidase/arylsulfatase and purified by SLW solid phase extraction column. The residues were detected for four β-agonists by UPLC-MS/MS.

    Results

    The proposed method showed a good linearity over the range of 0.1-20 μg/L for the four β-agonists with r>0.999 6 and the limit of detection (LOD) of 0.03-0.10 μg/kg (S/N=3). The average recoveries were 70.3%-97.1% and the relative standard deviations were 0.25%-4.5% when the samples were spiked at levels of 2, 5, and 10 μg/kg.

    Conclusion

    This highly effective, selective, and sensitive method is suitable for detecting β-agonists in meat products.

     

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