刘殊, 张敏, 徐肖倩, 董瑞娜, 戴红. DEHP染毒致青春期雄性大鼠的生殖毒性及维生素C、E的保护作用[J]. 环境与职业医学, 2016, 33(8): 780-784. DOI: 10.13213/j.cnki.jeom.2016.15613
引用本文: 刘殊, 张敏, 徐肖倩, 董瑞娜, 戴红. DEHP染毒致青春期雄性大鼠的生殖毒性及维生素C、E的保护作用[J]. 环境与职业医学, 2016, 33(8): 780-784. DOI: 10.13213/j.cnki.jeom.2016.15613
LIU Shu, ZHANG Min, XU Xiao-qian, DONG Rui-na, DAI Hong. Reproductive Toxicity Induced by Di(2-ethylhexy) Phthalate in Adolescent Male Rats and Protective Effects of Vitamin C and Vitamin E[J]. Journal of Environmental and Occupational Medicine, 2016, 33(8): 780-784. DOI: 10.13213/j.cnki.jeom.2016.15613
Citation: LIU Shu, ZHANG Min, XU Xiao-qian, DONG Rui-na, DAI Hong. Reproductive Toxicity Induced by Di(2-ethylhexy) Phthalate in Adolescent Male Rats and Protective Effects of Vitamin C and Vitamin E[J]. Journal of Environmental and Occupational Medicine, 2016, 33(8): 780-784. DOI: 10.13213/j.cnki.jeom.2016.15613

DEHP染毒致青春期雄性大鼠的生殖毒性及维生素C、E的保护作用

Reproductive Toxicity Induced by Di(2-ethylhexy) Phthalate in Adolescent Male Rats and Protective Effects of Vitamin C and Vitamin E

  • 摘要: 目的

    探讨邻苯二甲酸二(2-乙基)已酯(DEHP)对青春期雄性大鼠生殖毒性及其作用机制,以及维生素C、维生素E的保护作用。

    方法

    选择4周龄Wistar雄性大鼠随机分为6组(每组10只):对照组(玉米油),DEHP低、中、高染毒组750、1 500、3 000 mg(/kg·d),维生素C组(3 000 mg/kg DEHP+1%维生素C)及维生素E组(3 000 mg/kg DEHP+1%维生素E);DEHP灌胃染毒,维生素C和维生素E经自主饮水暴露,连续6周。处死大鼠并迅速剥离睾丸组织,计算脏器系数,测定睾丸脂质过氧化物,利用HE染色检测睾丸组织形态学改变,ELISA法检测睾丸组织促卵泡刺激素、睾酮、黄体生成素水平。

    结果

    高剂量睾丸脏器系数显著下降(P<0.01),光镜可见中和高染毒组睾丸组织均有不同程度损伤,生精小管萎缩、管腔内出现空泡样变化、生殖细胞数量减少、间质细胞大片聚集。与溶剂对照相比,DEHP高染毒组睾丸中脂质过氧化物和丙二醛含量下降(P<0.05);各DEHP染毒组总超氧化物歧化酶均有所下降(P<0.05);高染毒组卵泡刺激素、促卵泡生成素、睾酮均高于对照组(P<0.05);维生素C和E对DEHP染毒所致睾丸组织形态学损伤均有一定的改善;维生素E对睾丸组织脂质过氧化损伤有保护作用。

    结论

    DEHP可致青春期雄性大鼠睾丸生殖毒性,维生素C和维生素E对其所致损伤具有一定的保护作用。

     

    Abstract: Objective

    To explore the mechanisms of reproductive toxicity induced by di(2-ethylhexyl) phthalate(DEHP), as well as the protective effects of vitamin C and vitamin E.

    Methods

    Four-week-old Wistar rats were divided into six groups randomly(n=10): control(corn oil), low, medium, and high dose 750, 1 500, and 3 000 mg/(kg·d) DEHP, vitamin C(3 000 mg/kg DEHP+1% vitamin C), and vitamin E(3 000 mg/kg DEHP+1% vitamin E) groups. DEHP exposure by lavage were administered to the rats, while vitamin C and vitamin E were exposed through drinking water freely. After six weeks, the rats were sacrificed and testicular tissues were collected to calculate organ coefficient and determine lipid peroxidation activity. The morphology change was observed by HE staining, and follicle-stimulating hormone(FSH), testosterone(T), and luteinizing hormone(LH) levels in testicular were measured by ELISA.

    Results

    The testicular organ coefficient was decreased in the high dose group(P<0.01). Varying degrees of testicular damage were observed in the medium and high dose groups, showing seminiferous tubule atrophy, vacuoles within lumen samples, decreased germ cells, and clustered Leydig cells. Lipid peroxide and malondialdehyde were decreased in the high dose group compared with the control group(P<0.05); while total superoxide dismutase decreased in all DEHP groups(P<0.05). LH, FSH, and T were higher in the high dose group than those in the control group(P<0.05). Vitamin C and vitamin E improved the morphological damage induced by DEHP, and vitamin E protected testicular tissues from lipid peroxidation.

    Conclusion

    DEHP could induce oxidative damage in testicular tissues, and vitamin C and vitamin E might protect testicular from DEHP.

     

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