陈冉, 张海东, 杨飞飞, 王辉, 王瑞. 染矽尘大鼠结缔组织生长因子和Ⅱ型肺泡细胞表面抗原的表达[J]. 环境与职业医学, 2017, 34(1): 58-62. DOI: 10.13213/j.cnki.jeom.2017.16330
引用本文: 陈冉, 张海东, 杨飞飞, 王辉, 王瑞. 染矽尘大鼠结缔组织生长因子和Ⅱ型肺泡细胞表面抗原的表达[J]. 环境与职业医学, 2017, 34(1): 58-62. DOI: 10.13213/j.cnki.jeom.2017.16330
CHEN Ran, ZHANG Hai-dong, YANG Fei-fei, WANG Hui, WANG Rui. Expression of connective tissue growth factor and type Ⅱ alveolar cell surface antigen in silica treated rats[J]. Journal of Environmental and Occupational Medicine, 2017, 34(1): 58-62. DOI: 10.13213/j.cnki.jeom.2017.16330
Citation: CHEN Ran, ZHANG Hai-dong, YANG Fei-fei, WANG Hui, WANG Rui. Expression of connective tissue growth factor and type Ⅱ alveolar cell surface antigen in silica treated rats[J]. Journal of Environmental and Occupational Medicine, 2017, 34(1): 58-62. DOI: 10.13213/j.cnki.jeom.2017.16330

染矽尘大鼠结缔组织生长因子和Ⅱ型肺泡细胞表面抗原的表达

Expression of connective tissue growth factor and type Ⅱ alveolar cell surface antigen in silica treated rats

  • 摘要: 目的 探讨结缔组织生长因子(CTGF)与Ⅱ型肺泡细胞表面抗原(KL-6)在染矽尘大鼠血清、支气管肺泡灌洗液(BALF)及肺组织中的表达及意义。

    方法 将49只Wistar雄性大鼠随机分为对照组和染尘后7、14、21、28、60、90 d共7个组,每组7只,染尘组采用非气管暴露法灌注二氧化硅悬浮液(100 mg/mL),对照组以同样方法灌注生理盐水,取其肺组织并采集血清及BALF。采用免疫组织化学法测定肺组织中CTGF和KL-6的表达水平;采用酶联免疫吸附法(ELISA)检测血清及BALF中CTGF、KL-6的质量浓度。

    结果 免疫组化结果显示,染尘大鼠肺组织中CTGF和KL-6呈阳性表达,且染尘组的平均光密度值均高于对照组,差异有统计学意义(P < 0.05);染尘后随时间的延长,CTGF表达呈先增后降的趋势,在染尘后28 d达到最高峰,差异有统计学意义(P < 0.05);KL-6呈持续上升趋势,且差异有统计学意义(P < 0.05)。ELISA结果显示,染尘大鼠血清及BALF中CTGF质量浓度的变化趋势基本与其在肺组织中的一致,但是在染尘后21 d达到峰值;KL-6在血清及BALF中的变化也基本与肺组织中一致。

    结论 CTGF和KL-6与肺纤维化有密切的关系,在矽肺形成过程中发挥了作用。

     

    Abstract: Objective To study the expression of connective tissue growth factor (CTGF) and type Ⅱ alveolar cell surface antigen (KL-6) in serum, bronchoalveolar lavage fluid (BALF), and lung tissue of silica treated rats.

    Methods Male Wistar rats (n=49) were assigned randomly into control and exposure groups (7, 14, 21, 28, 60, and 90 d after exposure) with seven rats in each group. The exposure groups were instilled with silicon dioxide by non-trachea exposure method (100 mg/mL), and the control group was instilled with sterilized physiological saline. Lung tissues were dissected and serum and BALF were collected. The expression levels of CTGF and KL-6 in the lung tissue samples were determined by immunohistochemistry. The mass concentrations of CTGF and KL-6 in serum and BALF were measured by ELISA.

    Results According to the results of immunohistochemistry staining, the exposure groups showed positive CTGF and KL-6 expressions in lung tissues and higher average optical density values than the control group (P < 0.05). In the exposure groups, along with the time after dust exposure increasing, the expression of CTGF showed a pattern of increasing at first and then decreasing with a peak on 28 d (P < 0.05), while KL-6 showed a continuous increase (P < 0.05). The changes of mass concentrations of CTGF and KL-6 in serum and BALF were consistent with those in lung tissues, with the mass concentration of CTGF reaching its peak on 21 d after exposure.

    Conclusion CTGF and KL-6 may have a close relationship with pulmonary fibrosis and play a role in the development of silicosis.

     

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