宋冠仪, 梅勇, 商律, 姬晓宇, 刘佩佩, 宋世震. 高效液相色谱-氢化物发生-原子荧光联用技术测定饮用水中四种形态砷[J]. 环境与职业医学, 2017, 34(4): 350-353. DOI: 10.13213/j.cnki.jeom.2017.16615
引用本文: 宋冠仪, 梅勇, 商律, 姬晓宇, 刘佩佩, 宋世震. 高效液相色谱-氢化物发生-原子荧光联用技术测定饮用水中四种形态砷[J]. 环境与职业医学, 2017, 34(4): 350-353. DOI: 10.13213/j.cnki.jeom.2017.16615
SONG Guan-yi, MEI Yong, SHANG Lü, JI Xiao-yu, LIU Pei-pei, SONG Shi-zhen. Determination of four arsenic species in drinking water by high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry[J]. Journal of Environmental and Occupational Medicine, 2017, 34(4): 350-353. DOI: 10.13213/j.cnki.jeom.2017.16615
Citation: SONG Guan-yi, MEI Yong, SHANG Lü, JI Xiao-yu, LIU Pei-pei, SONG Shi-zhen. Determination of four arsenic species in drinking water by high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry[J]. Journal of Environmental and Occupational Medicine, 2017, 34(4): 350-353. DOI: 10.13213/j.cnki.jeom.2017.16615

高效液相色谱-氢化物发生-原子荧光联用技术测定饮用水中四种形态砷

Determination of four arsenic species in drinking water by high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry

  • 摘要: 目的 建立高效液相色谱-氢化物发生-原子荧光(HPLC-HG-AFS)联用技术测定生活饮用水中四种形态砷的方法。

    方法 通过高效液相色谱实现水中形态砷的分离,原子荧光进行定性及定量。对硼氢化钾质量分数、盐酸体积分数、氢氧化钠质量分数、缓冲液质量浓度和溶液pH值等实验条件进行优化,再运用到实际样本检测中。

    结果 三价砷As(Ш)、一甲基砷酸(MMA)、二甲基砷酸(DMA)和五价砷As(Ⅴ)的检出限分别为0.36、0.69、0.39和2.34μg/L,四种形态砷的标准曲线相关系数均大于0.999,平均加标回收率为95.7%~107.9%,相对标准偏差为0.23%~9.31%。

    结论 采用HPLC-HG-AFS技术测定饮用水中不同形态砷,检出限低,精密度好,结果准确可靠。

     

    Abstract: Objective To establish a method for determination of four arsenic species in drinking water by high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS).

    Methods Arsenic speciation in water was separated by high performance liquid chromatography, and qualitatively and quantitatively determined by atomic fluorescence spectrometry. Experimental conditions were optimized including potassium borohydride mass fraction, hydrochloric acid volume fraction, sodium hydroxide mass fraction, buffer concentration, and solution pH. Then the method was applied to actual water samples.

    Results The detection limits of arsenic (Ⅲ), monomethylarsenic acid (MMA), dimethylarsenic acid (DMA), and arsenic (Ⅴ) were 0.36, 0.69, 0.39, and 2.34 μg/L, respectively. All correlation coefficients were greater than 0.999. The standard recovery rates were 95.7%-107.9%. The relative standard deviations were 0.23%-9.31%.

    Conclusion HPLC-HG-AFS is featured by low detection limit and high precision with accurate and reliable testing results for determination of arsenic speciation in drinking water.

     

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