李雪婷, 于佳, 李百祥. 孕期和孕乳期西玛津染毒对F1代大鼠生殖发育的影响[J]. 环境与职业医学, 2017, 34(7): 598-603. DOI: 10.13213/j.cnki.jeom.2017.16627
引用本文: 李雪婷, 于佳, 李百祥. 孕期和孕乳期西玛津染毒对F1代大鼠生殖发育的影响[J]. 环境与职业医学, 2017, 34(7): 598-603. DOI: 10.13213/j.cnki.jeom.2017.16627
LI Xue-ting, YU Jia, LI Bai-xiang. Reproductive toxicity to F1 offspring rats by simazine exposure during maternal gestation and gestation-and-lactation[J]. Journal of Environmental and Occupational Medicine, 2017, 34(7): 598-603. DOI: 10.13213/j.cnki.jeom.2017.16627
Citation: LI Xue-ting, YU Jia, LI Bai-xiang. Reproductive toxicity to F1 offspring rats by simazine exposure during maternal gestation and gestation-and-lactation[J]. Journal of Environmental and Occupational Medicine, 2017, 34(7): 598-603. DOI: 10.13213/j.cnki.jeom.2017.16627

孕期和孕乳期西玛津染毒对F1代大鼠生殖发育的影响

Reproductive toxicity to F1 offspring rats by simazine exposure during maternal gestation and gestation-and-lactation

  • 摘要: 目的 探讨生命早期西玛津染毒对仔鼠的生殖毒性。

    方法 成年SD大鼠雌性90只,雄性45只,按照2:1进行合笼。选取状态良好的孕鼠80只,随机分为8组,在孕期和孕乳期通过灌胃染毒西玛津,分别设立对照组(3%质量分数淀粉溶液)和低、中、高剂量组(12.5、50.0、200.0mg/kg)。待各组孕鼠产仔时,记录孕鼠的产仔情况,包括产仔数、活仔数、胎仔重量;母鼠哺乳结束后处死。各组子代大鼠随机抽取20只(雌雄各半)继续饲养,待6个月后处死。称取亲代母鼠主要脏器及子代生殖器湿重,计算脏器系数。利用ELISA法测定仔鼠血清中性激素水平,经过统计分析,评价生命早期西玛津染毒对仔鼠的生殖毒性。

    结果 西玛津对活仔率及胎仔均重无明显的影响。孕期染毒中、高剂量组,及孕乳期染毒高剂量组仔鼠21 d存活率明显低于对照组(P < 0.05)。孕期染毒和孕乳期染毒中、高剂量组F1代雄鼠睾丸系数均明显低于相应的对照组(P < 0.05);低、中、高剂量的孕乳期染毒组F1代雄鼠睾丸系数均低于孕期(P < 0.05)。孕乳期高剂量组F1代雌鼠的卵巢系数低于对照组(P < 0.05)。两段染毒时期中,中、高剂量组F1代雄鼠睾酮均低于对照组(P < 0.05);孕乳期中、高剂量组F1代雄鼠的睾酮水平均低于孕期染毒组(P < 0.05)。两个染毒时期中,仅高剂量组F1代雌鼠血清17-β雌二醇水平低于各自对照组(P < 0.05);仅孕乳期染毒高剂量组F1代雌鼠的17-β雌二醇水平明显低于孕期染毒的水平(P < 0.05)。

    结论 子代在生命早期通过母体接触西玛津对其生殖发育具有一定的毒作用。

     

    Abstract: Objective To evaluate the reproductive toxicity to offspring rats induced by simazine exposure during early life stage.

    Methods A total of 90 female and 45 male SD rats were allowed to mate in stainless steel cages with two females and one male per cage. Eighty pregnant rats in good condition were selected and divided into eight groups. Four groups received different doses of simazine during gestation and another four groups did during gestation-and-lactation. Each four groups included 0 mg/kg (3% starch solution, control), 12.5 mg/kg (low dose), 50.0 mg/kg (middle dose), and 200.0 mg/kg (high dose) groups. Litter numbers, numbers of litters alive, and weight of offspring were recorded. The dams were neutralized after weaning. Twenty offspring rats each group (10 females and 10 males) were raised until six months old and euthanized. Then we weighed main organs of the mother rats and reproductive organs of the offspring rats to calculate organ coefficients. ELISA was used to determine the serum sex hormone le vels of offspring rats. The reproductive toxicity to offspring rats by simazine exposure during early life stage was evaluated through statistical analysis.

    Results There were no evident effects of simazine on the survival rate and weight of offspring. The 21-day survival rates of the middle and high dose groups' offspring with maternal gestational exposure and of the high dose groups' offspring with maternal gestational-and-lactational exposure were lower than those of corresponding control groups (P < 0.05). The testis organ coefficients of male offspring were significantly lower in the middle and high dose groups with maternal gestational exposure and gestational-andla ctational exposure than in the corresponding controls (P < 0.05); the testis organ coefficients of male offspring of three dose groups with gestational-and-lactational exposure were lower than those of corresponding groups with gestational exposure (P < 0.05). The ovary organ coefficient of female offspring of the high dose group with gestational-and-lactational exposure were lower than that of the control group (P < 0.05). For both exposure protocols, the testosterone levels of male offspring of the middle and high dose groups were lower than those of the corresponding control groups (P < 0.05); the levels of male offspring of the middle and high dose groups with gestational-and-lactational exposure were lower than corresponding groups with maternal gestational exposure (P < 0.05). For both exposure protocols, the 17-β estradiol levels of the high dose groups' female offspring were lower than those of corresponding control groups' (P < 0.05); only the 17-β estradiol level of female offspring of the high dose group with gestational-and-lactational exposure was lower than that with gestational exposure (P < 0.05).

    Conclusion Simazine exposure during early life stage can affect the reproductive development of offspring rats.

     

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