母鼠金雀异黄素干预对雄性子代大鼠肥胖的影响
Effect of maternal genistein intervention on obesity of male offspring rats
-
摘要:
目的 研究母鼠金雀异黄素干预对雄性子代大鼠肥胖的影响。
方法 选用性成熟雌性大鼠，受孕后在孕期及哺乳期间均给予不同剂量的金雀异黄素干预，并设立对照组。干预组母鼠采用添加金雀异黄素的饲料喂饲，剂量分别为3 600、1 200、400 mg/kg。子鼠断乳后采用未添加金雀异黄素的饲料喂饲，直至8周末。实验期间每周观察雄性子鼠的体重；实验结束后测定大鼠身长计算Lee's指数，留取脂肪组织，计算脂肪脏器系数，分离血清进行血脂、血糖、胰岛素和炎性因子的检测。
结果 实验8周末，与对照组相比：3 600 mg/kg组子鼠体重降低（P=0.028）、高密度脂蛋白升高（P=0.038)，3 600 mg/kg组和1 200 mg/kg子鼠组Lee's指数、总脂肪湿重、血糖水平降低（P=0.031，P=0.049；P=0.009，P=0.016；P=0.033，P=0.026)。实验组子鼠胰岛素、炎性因子未发生明显变化。
结论 孕期及哺乳期金雀异黄素干预，可以降低雄性子鼠肥胖的发生。
Abstract:Objective To examine the potential effect of maternal genistein intervention on obesity of male offspring rats.
Methods Mature female rats were given different doses of genistein (3 600, 1 200, and 400 mg/kg feed, respectively) during pregnancy and lactation. A control group fed without genistein was set up. Offspring rats after weaning were fed no-genistein diet for eight weeks. The body weight of male offspring rats was observed once a week during the experiment. Lee's index and ratio of fat/body weight were calculated after the experiment. Blood lipid, blood glucose, insulin, and inflammatory factors in serum samples were detected.
Results After eight weeks, compared with the control group, the male offspring rats following maternal 3 600 mg/kg genistein treatment showed reduced body weight (P=0.028) and increased high-density lipoprotein cholesterol (P=0.038); those following maternal 3 600 mg/kg and 1 200 mg/kg genistein treatment showed decreased Lee's index, total wet weight of fat, and blood glucose (P=0.031, P=0.049; P=0.009, P=0.016; P=0.033, P=0.026). No obvious changes in insulin and cytokines were found in the offspring rats with maternal genistein treatment.
Conclusion Maternal genistein intake during pregnancy and lactation could reduce the occurrence of offspring obesity.
下载:
