杨欢, 王素华, 苏鑫, 高艳荣. 亚砷酸钠对雄性大鼠炎症因子和氧化应激的影响[J]. 环境与职业医学, 2017, 34(6): 542-544. DOI: 10.13213/j.cnki.jeom.2017.16812
引用本文: 杨欢, 王素华, 苏鑫, 高艳荣. 亚砷酸钠对雄性大鼠炎症因子和氧化应激的影响[J]. 环境与职业医学, 2017, 34(6): 542-544. DOI: 10.13213/j.cnki.jeom.2017.16812
YANG Huan, WANG Su-hua, SU Xin, GAO Yan-rong. Effects of sodium arsenite on inflammatory cytokines and oxidative stress in male rats[J]. Journal of Environmental and Occupational Medicine, 2017, 34(6): 542-544. DOI: 10.13213/j.cnki.jeom.2017.16812
Citation: YANG Huan, WANG Su-hua, SU Xin, GAO Yan-rong. Effects of sodium arsenite on inflammatory cytokines and oxidative stress in male rats[J]. Journal of Environmental and Occupational Medicine, 2017, 34(6): 542-544. DOI: 10.13213/j.cnki.jeom.2017.16812

亚砷酸钠对雄性大鼠炎症因子和氧化应激的影响

Effects of sodium arsenite on inflammatory cytokines and oxidative stress in male rats

  • 摘要: 目的 探讨亚砷酸钠对雄性大鼠血清中炎症因子和氧化应激水平的影响。

    方法 将32只健康无特定病原级雄性SD大鼠按体重随机分为4组,分别为对照组(超纯水)和低(10 μg/L)、中(100 μg/L)、高(1 000 μg/L)剂量亚砷酸钠溶液染毒组。每组8只,自由饮水染毒28 d。采用酶联免疫吸附试验测定大鼠血清中粒细胞-巨噬细胞集落刺激因子(GM-CSF)、白细胞介素-10(IL-10)、还原型烟酰胺腺嘌吟二核苷酸磷酸(NADPH)氧化酶、活性氧簇酶(ROS)、总抗氧化能力和一氧化氮(NO)水平。

    结果 亚砷酸钠染毒组大鼠血清GM-CSF表达量显著高于对照组(P < 0.05),且1 000 μg/L组的GM-CSF水平最高;抑炎因子IL-10含量均低于对照组(P < 0.05);1 000 μg/L剂量组ROS表达水平高于对照组(P < 0.05);与对照组相比,各染毒组NO表达量升高(P < 0.05),且存在剂量-反应关系。

    结论 亚砷酸钠进入雄性大鼠机体后产生炎症损伤和氧化应激损伤,损伤机制可能与NO增多、IL-10降低有关。

     

    Abstract: Objective To investigate the effects of sodium arsenite on serum inflammatory cytokine and oxidative stress levels in male rats.

    Methods Thirty-two specific-pathogen-free healthy male SD rats were randomly divided into four groups: control group, low dose, medium dose, and high dose groups, with eight rats in each group. The three exposure groups were intoxicated by 10, 100, and 1 000 μg/L NaAsO2 in drinking water, respectively, while the control group was given ultrapure water for free drinking. All the rats were sacrificed after 28 days. The levels of granulocyte-macrophage colony stimulating factor (GM-CSF), interleukin-10 (IL-10), nicotinanmide adenine dinucleotide phosphate (NADPH) oxidase, reactive oxygen species (ROS), total antioxidant capacity, and nitric oxide (NO) were determined by enzyme-linked immunosorbent assay.

    Results Sodium arsenite exposed rats showed significantly higher GM-CSF expression levels than the control group (P < 0.05), and the 1 000 μg/L group had the highest GM-CSF expression level. The anti-inflammatory cytokine IL-10 levels were lower in all exposure groups than in the control group (P < 0.05). The 1 000 μg/L group showed a higher ROS level than the control group (P < 0.05). NO expression levels were increased in all exposure groups compared with the control group (P < 0.05), and there was a dose-response relationship.

    Conclusion Sodium arsenite exposure in male rats could lead to inflammatory injury and oxidative stress injury, and the mechanism may be related to elevated NO and reduced IL-10.

     

/

返回文章
返回