刘晓利, 刘芳盈, 孟超, 李平, 张殿平, 宋菁, 刘克俭. 氟染毒对大鼠皮肤成纤维细胞中SOST及DKK1蛋白表达的影响[J]. 环境与职业医学, 2017, 34(10): 914-917. DOI: 10.13213/j.cnki.jeom.2017.17156
引用本文: 刘晓利, 刘芳盈, 孟超, 李平, 张殿平, 宋菁, 刘克俭. 氟染毒对大鼠皮肤成纤维细胞中SOST及DKK1蛋白表达的影响[J]. 环境与职业医学, 2017, 34(10): 914-917. DOI: 10.13213/j.cnki.jeom.2017.17156
LIU Xiao-li, LIU Fang-ying, MENG Chao, LI Ping, ZHANG Dian-ping, SONG Jing, LIU Ke-jian. Effects of fluoride exposure on expression of SOST and DKK1 in rat skin fbroblasts[J]. Journal of Environmental and Occupational Medicine, 2017, 34(10): 914-917. DOI: 10.13213/j.cnki.jeom.2017.17156
Citation: LIU Xiao-li, LIU Fang-ying, MENG Chao, LI Ping, ZHANG Dian-ping, SONG Jing, LIU Ke-jian. Effects of fluoride exposure on expression of SOST and DKK1 in rat skin fbroblasts[J]. Journal of Environmental and Occupational Medicine, 2017, 34(10): 914-917. DOI: 10.13213/j.cnki.jeom.2017.17156

氟染毒对大鼠皮肤成纤维细胞中SOST及DKK1蛋白表达的影响

Effects of fluoride exposure on expression of SOST and DKK1 in rat skin fbroblasts

  • 摘要: 目的 观察氟染毒对体外培养新生大鼠皮肤成纤维细胞硬骨素(sclerostin,SOST)和Dickkopf-1(DKK1)蛋白表达的影响。

    方法 采用原代培养方法,将大鼠皮肤成纤维细胞分为对照组(0mg/L)和7个染氟剂量组(0.0001、0.001、0.01、0.1、1、10、20mg/L),分别在4个染氟时间段(24、48、72、96h)收集细胞培养上清液,应用ELISA法测定SOST和DKK1蛋白表达水平。

    结果 与染氟0 mg/L组相比,SOST表达量在染氟48 h的0.000 1、0.001、0.01 mg/L组,染氟72 h的1、10 mg/L组及染氟96 h的0.001、0.01、0.1、1 mg/L组均明显下降(P<0.05)。与染氟24 h组相比,SOST表达量在染氟48 h的0.01 mg/L组,染氟96 h的0.001、0.01、0.1、1、10 mg/L组均明显下降(P<0.05)。与染氟0 mg/L组相比,DKK1表达量在染氟48 h的0.001 mg/L组,染氟72 h的0.01、0.1、1、10、20 mg/L组及染氟96 h的0.01、0.1、1、10、20 mg/L组均明显下降(P<0.05)。与染氟24 h组相比,DKK1表达量在染氟48 h的0.000 1、0.001、0.01 mg/L组,染氟72 h的0.001、0.01、1、10、20 mg/L及染氟96 h的0.01、0.1、1、20 mg/L组均明显下降(P<0.05)。

    结论 氟可导致大鼠皮肤成纤维细胞SOST和DKK1蛋白表达下调。

     

    Abstract: Objective To observe the changes of protein expressions of sclerostin (SOST) and dickkopf-1 (DKK1) in fibroblasts of rat skin following fluoride exposure.

    Methods Rat skin fibroblasts were cultured with fluoride at different 0, 0.0001, 0.001, 0.01, 0.1, 1, 10, and 20mg/L. The expression le vels of SOST and DKK1 in cell culture supernatants were detected at four exposure time points (24, 48, 72, and 96h) by ELISA.

    Results The expression levels of SOST of the 0.000 1, 0.001, 0.01 mg/L fluoride groups at 48 h, the 1 and 10 mg/L fluoride groups at 72 h, and the 0.001, 0.01, 0.1, and 1 mg/L fluoride groups at 96 h were significantly lower than that of the 0 mg/L fluoride group (P < 0.05). The expression levels of SOST of the 0.01mg/L fluoride group at 48h and the 0.001, 0.01, 0.1, 1, and 10mg/L fluoride groups at 96 h were significantly lower than those of the groups at 24 h (P < 0.05). The expression levels of DKK1 of the 0.001 mg/L fluoride group at 48 h, the 0.01, 0.1, 1, 10, and 20 mg/L fluoride groups at 72 h, and the 0.01, 0.1, 1, 10, and 20 mg/L fluoride groups at 96h were significantly lower than that of the 0mg/L fluoride group (P < 0.05). The expression levels of DKK1 of the 0.0001, 0.001, 0.01 mg/L fluoride groups at 48 h, the 0.001, 0.01, 1, 10, and 20 mg/L fluoride groups at 72 h, and the 0.01, 0.1, 1, and 20 mg/L groups at 96 h were significantly lower than those of the groups at 24 h (P < 0.05).

    Conclusion Fluoride can cause down-regulation of expression of SOST and DKK1 in rat skin fibroblasts.

     

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