郭金霞, 李吴萍, 陈建茂, 周建, 汪岭, 童琳, 宋琦如. 高温联合紫外线2~4个月连续暴露对大鼠血清白介素-21水平的影响[J]. 环境与职业医学, 2017, 34(8): 712-717. DOI: 10.13213/j.cnki.jeom.2017.17200
引用本文: 郭金霞, 李吴萍, 陈建茂, 周建, 汪岭, 童琳, 宋琦如. 高温联合紫外线2~4个月连续暴露对大鼠血清白介素-21水平的影响[J]. 环境与职业医学, 2017, 34(8): 712-717. DOI: 10.13213/j.cnki.jeom.2017.17200
GUO Jin-xia, LI Wu-ping, CHEN Jian-mao, ZHOU Jian, WANG Ling, TONG Lin, SONG Qi-ru. Effects of continuous exposure to high temperature combined with ultraviolet radiation for 2-4 months on serum interleukin-21 levels in rats[J]. Journal of Environmental and Occupational Medicine, 2017, 34(8): 712-717. DOI: 10.13213/j.cnki.jeom.2017.17200
Citation: GUO Jin-xia, LI Wu-ping, CHEN Jian-mao, ZHOU Jian, WANG Ling, TONG Lin, SONG Qi-ru. Effects of continuous exposure to high temperature combined with ultraviolet radiation for 2-4 months on serum interleukin-21 levels in rats[J]. Journal of Environmental and Occupational Medicine, 2017, 34(8): 712-717. DOI: 10.13213/j.cnki.jeom.2017.17200

高温联合紫外线2~4个月连续暴露对大鼠血清白介素-21水平的影响

Effects of continuous exposure to high temperature combined with ultraviolet radiation for 2-4 months on serum interleukin-21 levels in rats

  • 摘要: 目的 探讨高温联合紫外线2~4个月连续暴露对大鼠血清白介素-21(IL-21)水平的影响。

    方法 选取体重为(200±10)g的健康成年SD大鼠112只,雌雄各半。随机分为对照组、高温组、紫外线组以及联合组(高温+紫外线),每组雌雄各14只,分笼饲养。在紫外灯(310 nm,72 μW/cm2)和(38±1)℃高温环境中每天暴露2 h,连续暴露2个月和4个月后分别处死,采用酶联免疫吸附试验对大鼠血清IL-21进行检测。

    结果 高温联合紫外线分别暴露2个月及4个月后,联合组雄性大鼠最终体重(428.57±12.94)、(476.86±13.14)g,体重增长量(183.29±11.74)、(306.86±13.81)g明显低于对照组最终体重:(493.57±17.12)、(587.00±11.53)g;体重增长量:(249.00±17.99)、(414.43±12.41)g(均P<0.05)。各组雌性大鼠体重差异均无统计学意义(P > 0.05)。暴露2个月后,各暴露组无论雄性、雌性大鼠的脾脏系数和胸腺系数均无差异(P > 0.05)。暴露4个月后,各暴露组雄性大鼠脾脏系数高温组:(1.311±0.037)‰;紫外线组:(1.270±0.058)‰;联合组:(1.197±0.024)‰均小于对照组(1.410±0.026)‰(P<0.05);高温组雄性大鼠胸腺系数(0.576±0.041)‰小于对照组(0.996±0.067)‰(P<0.05);而雌性大鼠的脏器系数均无差异(P > 0.05)。相同暴露时间和暴露因素,雌、雄大鼠间血清IL-21水平差异均无统计学意义(P>0.05),故将雌雄大鼠合并分析。除2个月紫外线组(487.69±15.21)ng/L外,其他各暴露组2个月:高温组(365.05±18.17)ng/L、联合组(278.66±11.70)ng/L;4个月:高温组(343.73±22.97)ng/L、紫外线组(264.45±23.96)ng/L、联合组(150.90±23.06)ng/L大鼠血清IL-21水平均低于对照组2个月(518.11±18.68)ng/L、4个月(582.18±28.90)ng/L(P<0.05);紫外线组和联合组大鼠暴露4个月时血清IL-21水平均低于暴露2个月时(P<0.01),无论暴露2个月还是4个月,联合组大鼠血清IL-21水平均明显低于高温组和紫外线组(P<0.05)。

    结论 高温联合紫外线暴露对大鼠体重增长量、免疫器官重量以及血清IL-21水平均有一定的影响,且随着暴露时间的延长,血清IL-21含量呈降低趋势。

     

    Abstract: Objective To study the effects of high temperature combined with ultraviolet for continuous 2-4 months on serum interleukin-21 (IL-21) levels in rats.

    Methods A total of 112 healthy adult SD rats, (200±10) g, half male and half female, were randomly divided into control group, high temperature group, ultraviolet group, and combined group (high temperature+ultraviolet), with 14 female and 14 male rats in each group and raised separately. The rats were correspondingly exposed to high temperature(38±1) ℃ and ultraviolet (310 nm, 72μW/cm2) for 2 h per day. After continuous exposure for 2 or 4 months, the rats were sacrificed and serum IL-21 was measured by ELISA.

    Results After being exposed to high temperature combined with ultraviolet for 2 months and 4 months, the final weight(428.57±12.94), (476.86±13.14) g and weight gain(183.29±11.74), (306.86±13.81) g of male SD rats were significantly lower than those of the control groupfinal weight:(493.57±17.12), (587.00±11.53) g; weight gain:(249.00±17.99), (414.43±12.41) g (all Ps < 0.05), respectively. No significant differences in body weight were found for female rats (P > 0.05). After continuous exposure for 2 months, no statistical difference was found in the spleen coefficients and thymus coefficients by all exposure treatments and by gender (P > 0.05). After continuous exposure for 4 months, the spleen coefficients of each exposed grouphigh temperature group:(1.311±0.037) ‰; ultraviolet group:(1.270±0.058) ‰; combined group:(1.197±0.024) ‰ were smaller than those of the control group(1.410±0.026) ‰ (P < 0.05); the thymus coefficient of male rats in the high temperature group(0.576±0.041) ‰ was lower than that of the control group(0.996±0.067) ‰ (P < 0.05); there was no difference in organ coefficients for the female rats administrated with different exposures (P > 0.05). Under the same exposure time and exposure treatments, there was no gender difference in serum IL-21 levels (P > 0.05); therefore, the female and male rats were merged for further analysis. Except the ultraviolet group after 2 months(487.69±15.21) ng/L, the levels of serum IL-21 in the other groups2 months:high temperature group, (365.05±18.17) ng/L, combined group, (278.66±11.70) ng/L; 4 months:high temperature group, (343.73±22.97) ng/L, ultraviolet group, (264.45±23.96) ng/L, combined group, (150.90±23.06) ng/L were significantly lower than those in the control group2 months:(518.11±18.68) ng/L; 4 months:(582.18±28.90) ng/L (P < 0.05). The serum IL-21 levels of the 4-month ultraviolet group and the combined group were lower than the corresponding values of the 2-month groups (P < 0.01). The level of serum IL-21 in the combined group was significantly lower than those of the high temperature group and the ultraviolet group treated for both 2 months and 4 months (P < 0.05).

    Conclusion The combination exposure of high temperature and ultraviolet may affect body weight gain, immune organ weight, and serum IL-21 levels of rats, and serum IL-21 levels would decrease with prolonged exposure time.

     

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