张雄, 宋肖肖, 肖霞, 吴洁, 徐庆华, 马瑞, 吕艳, 张超, 蹇秀桂, 李玉萍, 李燕. 农村妇女孕期全程拟除虫菊酯类农药暴露对脐血瘦素水平的影响[J]. 环境与职业医学, 2020, 37(4): 321-326. DOI: 10.13213/j.cnki.jeom.2020.19803
引用本文: 张雄, 宋肖肖, 肖霞, 吴洁, 徐庆华, 马瑞, 吕艳, 张超, 蹇秀桂, 李玉萍, 李燕. 农村妇女孕期全程拟除虫菊酯类农药暴露对脐血瘦素水平的影响[J]. 环境与职业医学, 2020, 37(4): 321-326. DOI: 10.13213/j.cnki.jeom.2020.19803
ZHANG Xiong, SONG Xiao-xiao, XIAO Xia, WU Jie, XU Qinghua, MA Rui, LÜ Yan, ZHANG Chao, JIAN Xiu-gui, LI Yu-ping, LI Yan. Impact of maternal exposure to pyrethroid pesticides across pregnancy on leptin in cord blood of pregnant women in rural areas[J]. Journal of Environmental and Occupational Medicine, 2020, 37(4): 321-326. DOI: 10.13213/j.cnki.jeom.2020.19803
Citation: ZHANG Xiong, SONG Xiao-xiao, XIAO Xia, WU Jie, XU Qinghua, MA Rui, LÜ Yan, ZHANG Chao, JIAN Xiu-gui, LI Yu-ping, LI Yan. Impact of maternal exposure to pyrethroid pesticides across pregnancy on leptin in cord blood of pregnant women in rural areas[J]. Journal of Environmental and Occupational Medicine, 2020, 37(4): 321-326. DOI: 10.13213/j.cnki.jeom.2020.19803

农村妇女孕期全程拟除虫菊酯类农药暴露对脐血瘦素水平的影响

Impact of maternal exposure to pyrethroid pesticides across pregnancy on leptin in cord blood of pregnant women in rural areas

  • 摘要: 背景

    瘦素可影响婴儿早期体重增长水平,而孕期拟除虫菊酯类农药暴露可影响新生儿出生体重。

    目的

    了解农村妇女孕期全程日常生活中拟除虫菊酯类农药暴露对胎儿脐血瘦素水平的影响。

    方法

    2015年1月-2018年7月,在云南宣威农村地区建立队列,自孕早期追踪至婴儿出生,共566对母婴纳入本研究。在妇女孕早、中、晚期进行问卷调查及晨尿收集,并采集新生儿脐血。使用高效液相色谱串联质谱法检测尿样拟除虫菊酯类农药代谢产物3-苯氧基苯甲酸(3-PBA)、4-氟-3-苯氧基苯甲酸(4-F-3-PBA)和二溴菊酸(DBCA)浓度,采用ELISA法检测脐血瘦素水平。根据3-PBA、4-F-3-PBA和DBCA检出水平将研究对象分为未检出组(< LOD)、低暴露组(LOD~检出值P50)、高暴露组(>检出值P50);根据3个孕期尿样的拟除虫菊酯类农药代谢产物检出与否,将研究对象分为3个孕期均未检出组、1个孕期检出组、2个孕期检出组、3个孕期均检出组。利用多元线性回归法分析孕期拟除虫菊酯类农药代谢产物暴露水平对脐血瘦素的影响。

    结果

    孕期3-PBA、DBCA和4-F-3-PBA的浓度中位数范围分别为0.413~0.459、0.964~1.431、0.310~0.394 μg·g-1(以肌酐计),瘦素浓度中位数为3.936 μg·L-1。孕晚期DBCA暴露水平与脐血瘦素浓度呈正相关b(95%CI)=0.006(0.001~0.010)。孕早期DBCA低暴露组水平(b=0.104,95%CI:0.015~0.194,P=0.023)和高暴露组水平(b=0.136,95%CI:0.005~0.266,P=0.042)与瘦素浓度呈正相关。4-F-3-PBA低暴露组孕中期水平(b=0.084,95%CI:0.001~0.167,P=0.048)和高暴露组孕早期(b=0.078,95%CI:0.006~0.150,P=0.034)、孕中期(b=0.114,95%CI:0.030~0.198,P=0.008)、孕晚期(b=0.092,95%CI:0.003~0.181,P=0.043)水平与脐血瘦素浓度呈正相关。1个孕期检出组、2个孕期检出组和3个孕期均检出组4-F-3-PBA的检出水平与瘦素浓度呈正相关(b=0.108,95%CI:0.028~0.189,P=0.008;b=0.096,95%CI:0.007~0.185,P=0.035;b=0.164,95%CI:0.051~0.277,P=0.004)。

    结论

    农村妇女孕期拟除虫菊酯类农药暴露在一定程度上可升高脐血瘦素水平。

     

    Abstract: Background

    Leptin can affect early-stage weight gain in infants, and pyrethroid pesticide exposure during pregnancy can affect birth weight in newborns.

    Objective

    This study aims to investigate the impact of maternal non-occupational exposure to pyrethroid pesticides during whole pregnancy on fetal cord blood leptin levels.

    Methods

    A total of 566 mother-infant pairs were included into current study from a prospective birth cohort in rural Xuanwei, Yunnan, China from January 2015 to July 2018. In each trimester of pregnancy the participated women were asked to complete a questionnaire survey and their urine samples were collected, and the cord blood samples of newborns were also collected after delivery. Pyrethroid metabolites such as 3-phenoxybenzoic acid (3-PBA), 4-fluoro-3-phenoxybenzoic acid (4-F-3-PBA), and (2, 2-dibromovinyl)-2, 2-dimethyl-cyclopropane-1-carboxylicacid (DBCA) in urine samples were measured by high performance liquid chromatography-tandem mass spectrometry. Leptin in cord blood samples was tested by ELISA. Based on the levels of 3-PBA, 4-F-3-PBA, and DBCA, the study subjects were divided into undetected group (< LOD), low exposure group (LOD-P50 of detected level), and high exposure group (>P50 of detected level); based on the detection state of pyrethroid metabolites in urine samples of three pregnant stages, the study subjects were divided into three groups:negative during pregnancy; positive in any one trimester; positive in any two trimesters; positive in all trimesters. Multiple linear regression analysis was used to explore the impacts of exposure levels of pyrethroid metabolites during pregnancy on leptin in cord blood.

    Results

    During whole pregnancy, the 3-PBA, DBCA, and 4-F-3-PBA median concentrations were 0.413-0.459, 0.964-1.431, and 0.310-0.394 μg·g-1 (in creatinine), respectively, and the leptin median concentration was 3.936 μg·L-1. DBCA exposure level in the third trimesterb (95%CI)=0.006 (0.001-0.010) was positively correlated with leptin concentration. BDCA low exposure level (b=0.104, 95%CI:0.015-0.194, P=0.023) and high exposure level (b=0.136, 95%CI:0.005-0.266, P=0.042) in the first trimester were positively correlated with leptin concentration. 4-F-3-PBA low exposure level in the second trimester (b=0.084, 95%CI:0.001-0.167, P=0.048) and high exposure level in the first (b=0.078, 95%CI:0.006-0.150, P=0.034), second (b=0.114, 95%CI:0.030-0.198, P=0.008), and third (b=0.092, 95%CI:0.003-0.181, P=0.043) trimesters were positively correlated with leptin concentration. The levels of 4-F-3-PBA in the groups with positive pyrethroid metabolites in any one trimester, any two trimesters, and three trimesters were positively correlated with leptin concentration (b=0.108, 95%CI:0.028-0.189, P=0.008; b=0.096, 95%CI:0.007-0.185, P=0.035; b=0.164, 95%CI:0.051-0.277, P=0.004).

    Conclusion

    Prenatal pyrethroid pesticide exposure among mothers in rural areas may increase the leptin levels in cord blood of fetuses.

     

/

返回文章
返回