陈子龙, 李海松, 管斯琪, 陈天帷, 王彬. 硫酸铵和硝酸铵对大鼠生精功能的影响[J]. 环境与职业医学, 2020, 37(9): 903-908. DOI: 10.13213/j.cnki.jeom.2020.20233
引用本文: 陈子龙, 李海松, 管斯琪, 陈天帷, 王彬. 硫酸铵和硝酸铵对大鼠生精功能的影响[J]. 环境与职业医学, 2020, 37(9): 903-908. DOI: 10.13213/j.cnki.jeom.2020.20233
CHEN Zi-long, LI Hai-song, GUAN Si-qi, CHEN Tian-wei, WANG Bin. Effects of ammonium sulfate and ammonium nitrate on spermatogenic dysfunction in rats[J]. Journal of Environmental and Occupational Medicine, 2020, 37(9): 903-908. DOI: 10.13213/j.cnki.jeom.2020.20233
Citation: CHEN Zi-long, LI Hai-song, GUAN Si-qi, CHEN Tian-wei, WANG Bin. Effects of ammonium sulfate and ammonium nitrate on spermatogenic dysfunction in rats[J]. Journal of Environmental and Occupational Medicine, 2020, 37(9): 903-908. DOI: 10.13213/j.cnki.jeom.2020.20233

硫酸铵和硝酸铵对大鼠生精功能的影响

Effects of ammonium sulfate and ammonium nitrate on spermatogenic dysfunction in rats

  • 摘要: 背景

    近年空气细颗粒物(PM2.5)的污染日益加剧,其成分复杂,具有多种细胞毒性作用,其中就包括生殖毒性。二次无机水溶性离子(SNA)如SO42-、NO3-和NH4+,是PM2.5的重要水溶性组分,研究SNA的生殖毒性意义重大。

    目的

    探讨硫酸铵及硝酸铵对于大鼠生精功能的损害作用及诱导大鼠生精障碍的染毒剂量。

    方法

    将96只3月龄健康雄性SD大鼠随机分为空白对照组和低、中、高剂量染毒组,每组24只。用硫酸铵、硝酸铵进行联合染毒,硫酸铵的低、中、高染毒剂量为147.5、295.0、442.5 mg·kg-1(以体重计),硝酸铵的低、中、高染毒剂量为178.5、357.5、536.5 mg·kg-1(以体重计)。以气管插管方式滴注染毒,空白对照组滴注相应体积的无菌生理盐水,染毒时间共6周。分别于实验第2周、4周、6周每组随机选取8只大鼠,观察其一般状况,取双侧睾丸、附睾,称重并计算脏器系数;观察睾丸病理改变,检测精液质量;取血清检测性激素水平。

    结果

    与空白对照组相比,中、高剂量组大鼠在染毒2周后出现蜷卧喜静,易激惹,体重较轻空白对照组体重为(316.50±12.15)g,中、高剂量组大鼠体重分别为(300.25±9.76)g、(287.24±14.53)g。睾丸组织HE染色结果显示:染毒4周后,与空白对照组相比,中、高剂量组大鼠出现生精小管结构受损,管腔内的支持细胞及精子缺失,形态和结构异常等病理改变,以上改变随着染毒时间的延长而加重,高剂量组的睾丸组织病理改变较中剂量组更明显。染毒4周后,高剂量组大鼠精子活力(44.75±1.89)%低于空白对照组(53.81±4.07)%(P < 0.05);染毒6周后,中、高剂量组大鼠的睾丸脏器系数分别为(4.67±0.34)‰、(3.97± 0.29)‰,精子计数分别为(52.63±4.75)×106 mL-1、(48.63±2.24)×106 mL-1,精子活力分别为(37.81±2.88)%、(32.25±1.99)%,均低于空白组(5.61±0.34)‰、(73.38±6.98)×106 mL-1、(52.56±2.99)%(P < 0.05)。染毒6周后,中、高剂量组大鼠卵泡刺激素(FSH)水平(11.32± 0.69)IU·L-1、(12.29±0.68)IU·L-1高于空白对照组(9.23±0.74)IU·L-1P < 0.05);高剂量组睾酮(T)水平(0.69±0.08)μg·L-1低于空白对照组(1.45±0.24)μg·L-1P < 0.05)。染毒6周后,中、高剂量组精子计数和精子活力均较2周及4周时有明显下降(P < 0.05);随着染毒时间增加,高剂量组T水平下降。

    结论

    硫酸铵和硝酸铵能够损伤雄性大鼠生精功能,以295.0 mg·kg-1硫酸铵、357.5 mg·kg-1硝酸铵对大鼠进行6周的气管插管滴注染毒可以诱导大鼠产生生精功能障碍。

     

    Abstract: Background

    In recent years, the pollution of fine particulate matters (PM2.5) has become increasingly serious. Its components are complex and have a variety of cytotoxic effects, including reproductive toxicity. Secondary inorganic water-soluble ions (SNA), such as SO42-, NO3- and NH4+, are important water-soluble components of PM2.5. It is significant to study the potential reproductive toxicity of SNA.

    Objective

    This experiment investigates the damage of ammonium sulfate and ammonium nitrate to spermatogenic function and their doses inducing spermatogenic dysfunction in rats.

    Methods

    Ninety-six healthy male SD rats aged 3 months were randomly divided into a blank control group and low, medium, and high dose exposure groups, with 24 rats in each group. Combined ammonium sulfate (147.5, 295.0, and 442.5 mg·kg-1, in body weight, respectively) and ammonium nitrate (178.5, 357.5, and 536.5mg·kg-1, in body weight, respectively) at designed doses were intratracheally instilled into the trachea of rats in the three exposure groups, and sterile normal saline at the same volume for the blank control group. The exposure lasted for six weeks. At week 2, 4, and 6, the general conditions of eight random rats in each group were observed; bilateral testis and epididymis were taken to calculate organ coefficients, observe pathological changes, and detect semen quality; and serum was collected to detect sex hormone levels.

    Results

    Compared with the blank control group, the rats in the medium and high dose exposure groups showed changes such as lying curled up and quiet, being more irritable, and lower weight after 2 weeks of exposurethe weights of the blank control group and the medium and high dose groups were (316.50±12.15) g, (300.25±9.76) g, and (287.24±14.53) g, respectively. The results of testicular tissues with HE staining showed that after 4 weeks of exposure, in the medium and high dose exposure groups, the spermatogenic tubules were structurally damaged, sertoli cells and sperms in the tubules were missing, and the morphology and structure of the tubules were abnormal, compared with the blank control group; such pathological changes were aggravated with the extension of exposure time, and the pathological changes of testicular tissues were more obvious in the high dose exposure group than in the medium dose exposure group. After 4 weeks of exposure, the sperm motility of rats in the high dose group(44.75±1.89)% was lower than that in the blank control group(53.81±4.07)%(P < 0.05). After 6 weeks of exposure, the testicular organ coefficients of the medium and high dose groups were (4.67±0.34)‰ and (3.97±0.29)‰, respectively; the sperm counts were (52.63±4.75)×106 mL-1 and (48.63±2.24)×106 mL-1, respectively; the sperm motilities were (37.81±2.88)% and (32.25±1.99)%, respectively; the above indices were reduced compared with the blank control group(5.61±0.34)‰, (73.38±6.98)×106 mL-1, and (52.56±2.99)% (P < 0.05). After 6 weeks of exposure, the levels of follicle stimulating hormone (FSH) in the medium and high dose groups(11.32±0.69) IU·L-1 and (12.29±0.68) IU·L-1 respectively were higher than that in the blank control group(9.23±0.74) IU·L-1 (P < 0.05); the testosterone (T) level in the high dose group(0.69±0.08) μg·L-1 was lower than that in the blank control group(1.45±0.24) μg·L-1 (P < 0.05). After 6 weeks of exposure, the sperm count and motility were significantly lower than those after 2 or 4 weeks (P < 0.05). With the increase of exposure time, the T levels in the high dose groups decreased.

    Conclusion

    Ammonium sulfate and ammonium nitrate can damage the spermatogenic function of male rats. Endotracheal intubation infusion of 295.0mg·kg-1 ammonium sulfate and 357.5mg·kg-1 ammonium nitrate for 6 weeks can induce spermatogenic dysfunction in rats.

     

/

返回文章
返回