袁志坚, 吴小瑜, 黄寅, 何文涓, 兰天. 恰玛古乙醇提取物对博来霉素致肺纤维化大鼠的作用及其机制[J]. 环境与职业医学, 2020, 37(10): 999-1004. DOI: 10.13213/j.cnki.jeom.2020.20319
引用本文: 袁志坚, 吴小瑜, 黄寅, 何文涓, 兰天. 恰玛古乙醇提取物对博来霉素致肺纤维化大鼠的作用及其机制[J]. 环境与职业医学, 2020, 37(10): 999-1004. DOI: 10.13213/j.cnki.jeom.2020.20319
YUAN Zhi-jian, WU Xiao-yu, HUANG Yin, HE Wen-juan, LAN Tian. Effect of Brassica rapa L. ethanol extract on bleomycin-induced pulmonary fibrosis in rats and its mechanism[J]. Journal of Environmental and Occupational Medicine, 2020, 37(10): 999-1004. DOI: 10.13213/j.cnki.jeom.2020.20319
Citation: YUAN Zhi-jian, WU Xiao-yu, HUANG Yin, HE Wen-juan, LAN Tian. Effect of Brassica rapa L. ethanol extract on bleomycin-induced pulmonary fibrosis in rats and its mechanism[J]. Journal of Environmental and Occupational Medicine, 2020, 37(10): 999-1004. DOI: 10.13213/j.cnki.jeom.2020.20319

恰玛古乙醇提取物对博来霉素致肺纤维化大鼠的作用及其机制

Effect of Brassica rapa L. ethanol extract on bleomycin-induced pulmonary fibrosis in rats and its mechanism

  • 摘要: 背景

    现代社会环境问题日益严重,环境中粉尘、残留农药以及过敏原等均会导致肺纤维化,目前没有能有效缓解肺纤维化进展的方法和药物,中医药对肺纤维化的治疗作用日益受到重视。

    目的

    研究恰玛古乙醇提取液对博来霉素致肺纤维化大鼠的治疗作用,初步探究其对肺纤维化的作用机制。

    方法

    将50只SD大鼠随机分为对照组、模型组、地塞米松组(1 mg·kg-1,腹腔注射)和恰玛古不同剂量组(25、50 g·kg-1,灌胃),除对照组外,其他各组动物均采用气管注射博来霉素(5mg·kg-1)建立大鼠肺纤维化模型,建模7d后开始给药,连续给药21d。观察大鼠体重变化;用大鼠肺功能仪检测大鼠的用力肺活量(FVC)、第1秒用力呼气容积(FEV1)、呼气流量峰值(PEF);检测大鼠肺系数;采用苏木精-伊红染色检测肺组织形态学变化;用免疫组织化学法检测肺组织中Ⅰ型胶原和Ⅲ型胶原表达情况;用酶联免疫吸附试验检测肺组织中Ⅰ型胶原、Ⅲ型胶原和炎症因子肿瘤坏死因子(TNF)-α、白介素(IL)-6的含量。

    结果

    与模型组相比,第28天时:50g·kg-1恰玛古组的大鼠体重明显增加(262.7±30.2)g vs(235.4±38.9)g,P < 0.05);25、50g·kg-1恰玛古组的大鼠FEV1、FVC、PEF有不同程度升高(6.15±0.28)、(6.81±0.36)mL vs(4.85±0.25)mL,(7.22±0.31)、(7.86±0.28)mL vs(5.92±0.23)mL,(30.61±8.88)、(32.14±6.27)mL vs(24.65±6.12)mL,P < 0.05),肺系数减小(0.58±0.09)%、(0.53±0.10)% vs(0.73±0.14)%,P < 0.05),肺组织内炎性细胞浸润减少,肺泡壁断裂及肺泡腔融合较少,肺纤维化程度减轻;50 g·kg-1恰玛古组的大鼠肺组织内Ⅰ型胶原和Ⅲ型胶原表达降低(2.15±0.56)mg·L-1 vs(2.82±0.55)mg·L-1,(2.35±0.58)mg·L-1 vs(3.02±0.59)mg·L-1P < 0.05);25、50 g·kg-1恰玛古组的大鼠肺组织内炎症因子TNF-α、IL-6表达降低(397.59±73.73)、(363.65±66.06)ng·L-1 vs(554.59±115.81)ng·L-1,(194.71±37.74)、(166.20±28.33)ng·L-1 vs(253.39±72.14)ng·L-1P < 0.05)。

    结论

    恰玛古可能是通过抑制Ⅰ型胶原、Ⅲ型胶原和炎症因子TNF-α、IL-6含量,改善博来霉素导致的大鼠肺纤维化病变进程。

     

    Abstract: Background

    Environmental problems are increased dramatically in modern society. For example, dust, residual pesticides, and allergens can cause pulmonary fibrosis. At present, there are no effective methods and drugs to alleviate the progression of pulmonary fibrosis. Traditional Chinese medicine has received increasing attention for its potential therapeutic effect on the disease.

    Objective

    This experiment tests the therapeutic effect of Brassica rapa L. extract on pulmonary fibrosis induced by bleomycin, and explores relevant mechanism.

    Methods

    Fifty SD rats were randomly divided into a control group, a model group, a dexamethasone group (1 mg·kg-1, intraperitoneal injection), and two Brassica rapa L. groups (25 and 50 g·kg-1, intragastrical administration). Except the control group, the other groups of rats were transtracheally injected with bleomycin (5 mg·kg-1) to establish a pulmonary fibrosis model. After 7 d, intraperitoneal injection of dexamethasone and intragastric administration of Brassica rapa L. extract lasted for 21 d. Body weight changes were observed. Rat lung forced vital capacity (FVC), forced expiratory volume in one second (FEV1), and peak expiratory flow (PEF) were measured with a rat pulmonary function meter. Lung coefficient was calculated. Histomorphological changes of lung were evaluated after HE staining. The expressions of collagenⅠand collagen Ⅲ in lung tissues were detected after immunohistochemical staining. The contents of collagenⅠ, collagen Ⅲ, and inflammatory factorstumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) in lung tissues were detected by enzymelinked immunosorbent assay.

    Results

    Compared with the model group, on day 28, the body weight of rats in the 50 g·kg-1 Brassica rapa L. group was increased significantly(262.7±30.2) g vs (235.4±38.9) g, P < 0.05; the FVC, FEV1, and PEF of rats in the 25 and 50 g·kg-1 Brassica rapa L. groups were increased(6.15±0.28), (6.81±0.36) mL vs (4.85±0.25) mL; (7.22±0.31), (7.86±0.28) mL vs (5.92±0.23) mL; (30.61±8.88), (32.14±6.27) mL vs (24.65±6.12) mL, P < 0.05, accompanied with decreased lung coefficient(0.58±0.09)%, (0.53±0.10)% vs (0.73±0.14)%, P < 0.05), reduced inflammatory cell infiltration in lung tissues, less rupture of alveolar wall and fusion of alveolar cavity, and alleviated pulmonary fibrosis; the expression levels of collagen Ⅰ and collagen Ⅲ in lung tissues of rats in the 50 g·kg-1 Brassica rapa L. group were decreased(2.15±0.56) mg·L-1 vs (2.82±0.55) mg·L-1, (2.35±0.58) mg·L-1 vs (3.02±0.59) mg·L-1, P < 0.05; the expression levels of inflammatory factors TNF-α and IL-6 in lung tissues of rats in the 25 and 50 g·kg-1 Brassica rapa L. groups were decreased(397.59±73.73), (363.65±66.06) ng·L-1 vs (554.59±115.81) ng·L-1; (194.71±37.74), (166.20±28.33)ng·L-1 vs (253.39±72.14) ng·L-1, P < 0.05.

    Conclusion

    Brassica rapa L. may alleviate the pathological process of pulmonary fibrosis caused by bleomycin in rats via inhibiting the fibrosis caused by collagenⅠ, collagen Ⅲ, and the inflammatory factors TNF-α and IL-6.

     

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