吴立梦, 滕峥, 崔晓青, 王葳, 狄弘玮, 匡小舟, 任宏, 刘弘, 袁政安, 张曦, 林庆能. 上海市贝类水产品诺如病毒污染状况及基因分型[J]. 环境与职业医学, 2021, 38(8): 888-893. DOI: 10.13213/j.cnki.jeom.2021.21121
引用本文: 吴立梦, 滕峥, 崔晓青, 王葳, 狄弘玮, 匡小舟, 任宏, 刘弘, 袁政安, 张曦, 林庆能. 上海市贝类水产品诺如病毒污染状况及基因分型[J]. 环境与职业医学, 2021, 38(8): 888-893. DOI: 10.13213/j.cnki.jeom.2021.21121
WU Limeng, TENG Zheng, CUI Xiaoqing, WANG Wei, DI Hongwei, KUANG Xiaozhou, REN Hong, LIU Hong, YUAN Zheng'an, ZHANG Xi, LIN Qingneng. Norovirus contamination and genotypes in shellfish in Shanghai[J]. Journal of Environmental and Occupational Medicine, 2021, 38(8): 888-893. DOI: 10.13213/j.cnki.jeom.2021.21121
Citation: WU Limeng, TENG Zheng, CUI Xiaoqing, WANG Wei, DI Hongwei, KUANG Xiaozhou, REN Hong, LIU Hong, YUAN Zheng'an, ZHANG Xi, LIN Qingneng. Norovirus contamination and genotypes in shellfish in Shanghai[J]. Journal of Environmental and Occupational Medicine, 2021, 38(8): 888-893. DOI: 10.13213/j.cnki.jeom.2021.21121

上海市贝类水产品诺如病毒污染状况及基因分型

Norovirus contamination and genotypes in shellfish in Shanghai

  • 摘要: 背景

    诺如病毒(NoV)是影响食品卫生与安全的重要污染物。贝类可富集环境水体中的病毒,是重要的食源性病原体传播载体。

    目的

    了解NoV在上海市贝类水产品中的污染状况,并对阳性株进行鉴定分型,为预防食源性疾病的发生,制定防控措施提供参考依据。

    方法

    采用实时荧光逆转录聚合酶链式反应法对2017—2019年上海地区采集的580份贝类水产品中NoV进行检测,用逆转录巢式聚合酶链式反应法对部分阳性样本病毒衣壳蛋白(VP)1区扩增片段进行分型分析。

    结果

    580份贝类样品中NoV检出率为8.97%,其中NoV GⅠ型检出率为2.24%(13/580),NoV GⅡ型检出率为5.86%(34/580),NoV GⅠ和GⅡ混合型检出率为0.86%(5/580)。四个季节的贝类水产品中NoV检出率的差异有统计学意义(χ2=8.132,P=0.043),冬春季较高;不同种类贝类水产品NoV检出率差异有统计学意义(χ2=14.152,P=0.049),以蚌科、牡蛎科为高。VP1区部分核酸序列分子系统进化分析显示,贝类水产品中可检测到NoV GⅡ.2、3c、13、17共4个NoV GⅡ型的基因亚型存在。1株GⅡ.2型与参考株X81879同源性为88.8%;2株GⅡ.3c型之间同源性为91.6%,与参考株AB385626同源性为87.1%~92.0%;1株GⅡ.13型与参考株AY113106同源性为93.6%;1株GⅡ.17型与参考株LC037415同源性为98.6%,与2014年发现的江苏南京株KU757049和上海株KT380915同源性分别为97.8%和97.4%。

    结论

    上海地区贝类水产品中存在NoV污染,且污染具有季节特点,以冬春季污染较严重;其基因型以GⅡ型为主。

     

    Abstract: Background

    Norovirus (NoV) is a major cause of foodborne diseases. Shellfish as an important vector can bioconcentrate viruses from the environment.

    Objective

    This study investigates NoV contamination in retail shellfish in Shanghai and genotypes of positive strains, aiming to provide evidence to prevent foodborne diseases and make relevant preventive measures.

    Methods

    Real-time reverse transcription polymerase chain reaction (RT-PCR) was used for screening NoV in 580 shellfish samples collected from Shanghai markets from 2017 to 2019. Reverse transcription nested polymerase chain reaction (RT-Nest-PCR) was used for amplification of partial viral protein 1 (VP1) fragments and genotyping.

    Results

    Among a total of 580 shellfish samples, the positive rate of NoV was 8.97%. GⅡ was the main genotypes (5.86%, 34/580), followed by GⅠ (2.24%, 13/580) and GⅠ+GⅡ dural infection (0.86%, 5/580). The positive rates of NoV in shellfish samples were different among different seasons (χ2=8.132, P=0.043), higher in winter and spring. The positive rates in different shellfishes were also different (χ2=14.152, P=0.049), higher in Unionidae and Ostreidae. The phylogenetic analysis results for the partial VP1 showed that GⅡ.2, 3c, 13, and 17 were positive in the shellfish samples. The identity of nucleotide sequences between one GⅡ.2 strain and the reference X81879 was 88.8%; between two GⅡ.3c strains, 91.6% identity, and between the two GⅡ.3c strains and the reference AB385626, 87.1%-92.0% identity; between one GⅡ.13 strain and the reference AY113106, 93.6% identity; between one GⅡ.7 strain with the reference LC037415, Nanjing GⅡ.17 strain KU757049, and Shanghai GⅡ.17 strain (KT380915), the identities were 98.6%, 97.8%, and 97.4%, respectively.

    Conclusion

    NoV contamination is prevalent in retail shellfish in Shanghai, and displays seasonality with a winter/spring peak. NoV GⅡ remain the predominant genotype.

     

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