杨光红, 李军, 张爱华, 张雄飞, 姚慧, 杨远菊. 运用TK基因突变试验及小鼠微核试验测定95%乙草胺原药的遗传毒性[J]. 环境与职业医学, 2010, 27(12): 742-744.
引用本文: 杨光红, 李军, 张爱华, 张雄飞, 姚慧, 杨远菊. 运用TK基因突变试验及小鼠微核试验测定95%乙草胺原药的遗传毒性[J]. 环境与职业医学, 2010, 27(12): 742-744.
YANG Guang-hong , LI Jun , ZHANG Ai-hua , ZHANG Xiong-fei , YAO Hui , YANG Yuan-ju . Detection of Genetic Toxicity of 95% Acetochlor by TK Gene Mutation Test and Mice Micro-nucleus Test[J]. Journal of Environmental and Occupational Medicine, 2010, 27(12): 742-744.
Citation: YANG Guang-hong , LI Jun , ZHANG Ai-hua , ZHANG Xiong-fei , YAO Hui , YANG Yuan-ju . Detection of Genetic Toxicity of 95% Acetochlor by TK Gene Mutation Test and Mice Micro-nucleus Test[J]. Journal of Environmental and Occupational Medicine, 2010, 27(12): 742-744.

运用TK基因突变试验及小鼠微核试验测定95%乙草胺原药的遗传毒性

Detection of Genetic Toxicity of 95% Acetochlor by TK Gene Mutation Test and Mice Micro-nucleus Test

  • 摘要: 目的 观察95%乙草胺原药的遗传毒性,为其安全使用及农药管理登记提供依据。

    方法 建立95%乙草胺原药的致突变试验模型,通过TK基因突变频率和微核率对其遗传毒性进行评价。

    结果 95%乙草胺原药各剂量组TK6细胞TK位点突变频率与阴性对照组比较,差异均无统计学意义(P > 0.05);与阴性对照组比较, 95%乙草胺原药染毒组的骨髓嗜多染红细胞微核率未见明显增高(P > 0.05)。

    结论 在本试验条件下, 95%乙草胺原药无诱导小鼠骨髓细胞染色体损伤及致TK6细胞基因突变的作用。

     

    Abstract: Objective To observe the genetic toxicity of 95% acetochlor, in order to provide scientific basis for its safe use, registered pesticide management and control measures.

    Methods Mutagenicity experimental models were set up. Genetic toxicity of 95% acetochlor was evaluated with TK gene mutation frequency and micronucleus rate.

    Results No significant difference existed between every experimental group and negative control group for TK gene mutation frequency(P > 0.05). Compared with negative control group, the micronuleus rates of mice didn't show a statistical increase in every experimental group (P > 0.05).

    Conclusion Under this experimental condition, 95% acetochlor cannot induce chromosome injury of mice polychromatic erythrocytes and gene mutation of TK6 cell.

     

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