水杨酸钠对百草枯所致大鼠肺组织核转录因子和炎性因子的影响
Mechanism of Sodium Salicylate on Paraquat-induced Effects of NF-κB, TNF-α, and TGF-β1 in Rat Lung
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摘要:
目的 探讨水杨酸钠(sodium salicylate,NaSAL)对抗百草枯(paraquat,PQ)致肺损伤的作用机制。 方法 将72 只雄性SD大鼠随机分为溶剂对照组(生理盐水灌胃)、PQ染毒组和PQ+NaSAL 干预组。PQ经口染毒,剂量为80 mg/kg(按大鼠体重),干预组给予染毒组相等剂量PQ后立即腹腔注射NaSAL(120 mg/kg)。在染毒后第1、3、7、14 天取对照组、染毒组、干预组各6 只动物进行检查,分别用凝胶电泳迁移率实验(EMSA)和酶联免疫吸附法(ELISA)检测肺组织中核转录因子(NF-κB)活性变化及肿瘤坏死因子(TNF-α)、转化生长因子-β1 (TGF-β1)蛋白含量的改变。 结果 染毒组大鼠肺组织中NF-κB 活性增高;肺组织中TNF-α、TGF-β1 蛋白表达增强,各时点蛋白水平与对照组相比差异有统计学意义(P<0.05)。干预组NF-κB活性较染毒组降低;与染毒组相比,干预组肺组织TNF-α、TGF-β1 蛋白水平表达明显下降,相应时点差异也有统计学意义(P<0.05)。 结论 NaSAL 干预后可以降低肺组织中NF-κB的活性,减少TNF-α、TGF-β1 的蛋白表达,提示NaSAL 可以改善PQ中毒引起的肺损伤。 Abstract:Objective To investigate the potential protective mechanism of sodium salicylate(NaSAL)on the pulmonary damage induced by paraquat(PQ)in rats. Methods We established rat poisoning model by intragastric administration of 80mg/kg PQ. A total of 72 male SD rats were randomly divided into control group, PQ treated group and PQ plus NaSAL intervention group. Control group were orally administered with an injection of 0.9% NaCl at the volume of 0.005 mL/g of body weight; PQ treated group were made 80 mg/kg PQ orally; PQ plus NaSAL group were given an intraperitoneal injection of 120 mg/kg NaSAL after an orally administration of 80 mg/kg PQ. Animals were sacrificed in batches on days 1, 3, 7, 14 after experiment. Then, the level of nuclear factor of kappa B(NF-κB)was assayed by electrophoresis mobility shift assay(EMSA), while the protein levels of tumor necrosis factor-α(TNF-α), transformin growth factor-β1(TGF-β1)were determined by enzyme-linked immunosorbent assay (ELISA). Results After 80 mg/kg PQ exposure, the levels of TGF-β1, TNF-α protein expression and the rat lung tissue NF- κB activity increased more remarkably than that of the control group(P<0.05). In comparison with PQ treated group, with the treatment of NaSAL(120 mg/kg), the level of TNF-α, TGF-β1 protein expression and the rat lung tissue NF-κB activity decreased significantly(P<0.05)and trended to normal values. Conclusion Treatment of NaSAL could decrease the level of TNF-α, TGF-β1 protein expression and the rat lung tissue NF-κB activity. It suggested that NaSAL could alleviate paraquat-induced pulmonary damage in rats to some extent.
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