桦褐孔菌多糖对二乙基亚硝胺致肝脏损伤的保护作用
The Protective Effect of Inonotus Obliquus Polysaccharide (IOP) on the Hepatic Injury Induced by Diethylnitrosamine
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摘要:
目的 研究桦褐孔菌多糖(inonotus obliquus polysaccharide)对二乙基亚硝胺(DEN)致肝脏损伤的保护作用。 方法 将50只小鼠随机分为5组:阴性对照组(蒸馏水),DEN组(20 mg/kg),桦褐孔菌多糖高(600 mg/kg)、中(300 mg/kg)、低(150 mg/kg)剂量组。阴性对照组及桦褐孔菌3 个剂量组每日灌胃,同时DEN组和桦褐孔菌3 个剂量组隔日腹腔注射DEN,持续5 周。采用HE染色法观察动物肝细胞核分裂相及枯否细胞数;采用酶动力学法测定肝组织匀浆上清液中谷胱甘肽-S-转移酶(GSTs)和微量丙二醛(MDA)含量;采用酶联免疫吸附法(ELISA)检测肝组织匀浆上清液中肿瘤坏死因子α(TNF-α)含量。 结果 DEN组肝组织中核分裂相和枯否细胞数明显多于阴性对照组(P<0.01),桦褐孔菌多糖高剂量组肝组织中核分裂相和枯否细胞数显著低于DEN组(P<0.05)。DEN组肝组织中GSTs 活性明显低于阴性对照组(P<0.05),MDA和TNF-α 含量明显高于阴性对照组(P<0.01 或P<0.05)。桦褐孔菌多糖高剂量组和中剂量组肝组织中GSTs 活性明显高于DEN组(P<0.01),3 个剂量组肝组织中MDA含量均显著低于DEN组(P<0.01 或P<0.05),只有高剂量组肝组织中TNF-α 含量明显低于DEN组(P<0.05)。 结论 桦褐孔菌多糖对DEN致肝脏损伤有一定的保护作用。 Abstract:Objective To study the preventive effect of inonotus obliquus polysaccharide(IOP)on the hepatic injury induced by diethylnitrosamine(DEN). Methods Fifty mice were randomly divided into five groups:negative control group(Distilled water), DEN group(20 mg/kg), high dose of IOP group(600 mg/kg), middle dose of IOP group(300 mg/kg), and low dose of IOP group(150 mg/ kg). Mice of negative control group were given with distilled water and the three IOP groups were given with IOP, all by gavage daily for 5 weeks. Mice of DEN group and three IOP groups were given with DEN intraperitoneally every other day. After 5 weeks, the mice were killed, the number of liver cell mitosis and Kupffer cell were counted under microscope with HE staining. The activity of glutathione S-transferases (GSTs)and content of malondialdehyde(MDA)were determined by enzyme dynamic method and the content of tumor necrosis factor α (TNF-α)in liver tissue was determined by ELISA. Results The numbers of mitosis and Kuffer cell in DEN group were more than that of the negative control group with significant statistical difference(P<0.01), and the high dose of IOP group showed less mitosis and Kuffer cell than that of the DEN group with significant statistical difference(P<0.05). The activity of GSTs in DEN group was lower than that of the negative control group with significant statistical difference(P<0.05), the contents of MDA and TNF-α in DEN group were higher than that of the negative control group with significant statistical difference(P<0.01 or P<0.05). The activities of GSTs in the high dose and middle dose of IOP group were higher than that of the DEN group with significant statistical difference(P<0.01). The contents of MDA in three different dose of IOP groups were lower than that of DEN group with significant statistical difference(P<0.01 or P<0.05). Only in high dose of IOP group the content of TNF-α was significantly lower than that of DEN group with significant statistical difference(P<0.05). Conclusion IOP has some protective effect on the hepatic injury induced by DEN.
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