慢性应激刺激致大鼠抑郁过程中红细胞免疫功能和T淋巴细胞亚群的变化
Changes of Erythrocyte Immune Function and T-lymphocyte Subsets in the Process of Stress-induced Depression in Rats
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摘要:
目的 观察慢性应激刺激致大鼠抑郁过程中红细胞免疫功能和T淋巴细胞免疫功能的动态变化以及指标间的相关性,探讨其在应激致抑郁发生、发展过程中的变化及其意义。 方法 通过慢性轻度不可预知性应激刺激Wistar大鼠,以行为学、液体消耗和血浆皮质酮的动态测定进行检测;采用酵母菌花环法、流式细胞术分别测定在各时点的红细胞各花环率和T淋巴细胞亚群变化。 结果 (1)应激组水平运动、垂直运动得分,清洁动作次数,糖水消耗和糖水偏爱百分率比对照组下降(P<0.05);血浆皮质酮水平增高(P<0.05)。(2)各时点应激组大鼠红细胞C3b受体花环率(RBC-C3bRR)均低于对照组(P<0.05);红细胞免疫复合物花环率(RBC-ICR)增高(P<0.05);T淋巴细胞亚群异常(P<0.05)。(3)红细胞免疫功能与T淋巴细胞免疫功能各指标间存在相关性。 结论 慢性轻度应激可诱发实验大鼠较长时间的行为及活动习性变化而导致抑郁形成,各免疫系统受损可能促使抑郁形成。 Abstract:Objective To observe the changes of erythrocyte immune functions and T-lymphocyte subsets in rats under stress-induced depression and explore the mechanism in the process. Methods Depression model was established by using chronic unpredictable mild stress (CUMS) stimulation combined with lonely raising. Weight and behavior changes were observed, plasma le vels of cortisone were measured by radioimmune assay, and then erythrocyte immune function, T-lymphocyte subsets were measured by the method of yeast rosette and flow cytometry respectively in different time point. Results (1)Compared with control group, the crossing and rearing movement times, clean times, the volume of sucrose-intake and the preference of sucrose in model group were decreased (P<0.05), whereas plasma corticosterone levels increased (P<0.05). (2) Compared with control group, the rate of RBC-C3bRR was decreased in every time point, but RBC-ICR was increased (P<0.05). The percent of T-lymphocyte appeared some alterations (P<0.05) in model group. (3) There were some relationships among these parameters. Conclusion The repeated chronic unpredictable mild stress stimulation can induce long-time changes in acts and activities of rats as depression. Impairment of immune functions such as erythrocyte immune functions and T-lymphocyte subsets might promote the induction of depression.
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