全氟辛烷磺酸钾对胎鼠肝脏信号因子STAT3蛋白表达的影响
Study on the Effects of PFOS-K on the Expression of STAT3 Protein in Embryo of Mice
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摘要:
目的 以胎鼠肝脏细胞中的信号转导及转录激活因子3(STAT3)蛋白表达的阳性细胞率(STAT3蛋白表达量)为指标,探讨全氟辛烷磺酸钾(PFOS-K)对小鼠胚胎的肝脏发育是否有影响,进而了解其胚胎毒作用。 方法 用染毒剂量分别为3、12 mg/kg (按体重计)两个浓度的PFOS-K混悬液给怀孕9 d的雌鼠灌胃,4 d后(即怀孕第13.5天时)采用免疫组织化学法检测胎鼠肝脏的STAT3蛋白表达量。 结果 3、12 mg/kg两个剂量组的阳性细胞表达率分别为41.62%和14.77%,差异有统计学意义(P<0.01)。 结论 PFOS-K能够影响小鼠胚肝脏发育过程中的信号转导因子STAT3蛋白的表达量,对哺乳动物的模式动物胎鼠肝脏细胞具有一定的胚胎毒作用。 Abstract:Objective To study the effect of perfluorooctane sulfonate (PFOS-K)on the liver development in embryo of mice and the toxicity in the embryo. Methods PFOS-K solution with concentrations of 3mg/kg and 12 mg/kg in a cumulative dose series was infused through gavage into nine-day-pregnant mice. Then, 4 days after the treatment the expression of signal transduction and activators of transcription 3(STAT3) protein in the embryo liver were measured by immunohistochemistry. Results The expression rates of SATA3 protein in mice with dose 3 mg/kg and 12 mg/kg were 41.62% and 14.77%, respectively. The results showed significant difference (P<0.01). Conclusion The results showed that PFOS-K could affect the expression amount of STAT3 protein in the embryo liver development. It could be concluded that PFOS-K has certain damage to the liver cell in embryo of mice.
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