贵州省苗族及布依族人群CYP1A1*2C基因多态性研究
CYP1A1*2C Gene Polymorphisms in Miao, Buyi Populations of Chinese Guizhou Province
-
摘要:
目的 检测贵州省苗族及布依族正常人群细胞色素氧化酶1A1*2C(CYP1A1*2C)基因多态性。 方法 采用Taqman-MGB探针,通过实时定量PCR (real-time PCR)对贵州省三都县125名苗族及122名布依族人群的血液样本进行CYP1A1*2C基因多态性分析,并采用χ2检验比较两民族该基因分布的差异。 结果 CYP1A1*2C野生纯合子(基因型AA)、突变杂合子(基因型AG)、突变纯合子(基因型GG)在苗族及布依族中基因型频率分别为65.6%、28.0%、6.4%及68.9%、25.4%、5.7%;A和G在苗族及布依族中基因频率分别为79.6%、20.4%及81.6%、18.4%,两民族人群的基因多态性分布差异无统计学意义。 结论 中国贵州省苗族及布依族人群中CYP1A1*2C基因型频率分布没有明显不同。 Abstract:Objective To investigate the gene polymorphisms of CYP1A1*2C in Chinese Miao, Buyi nationalities. Methods Taqman-MGB probe was used in real-time RealTime-PCR to test the polymorphisms of CYP1A1*2C in Chinese Miao(125), Buyi(122)people, and the significant difference between Miao and Buyi was checked by χ2 test. Results The distribution of wild homozygote(genetic type AA), mutant heterozygote(genetic type AG), mutant homozygote(genetic type GG)of CYP1A1*2C in Chinese Miao, Buyi populations were 65.6%, 28.0%, 6.4% and 68.9%, 25.4%, 5.7%, respectively. The distribution of A and G allele frequency of CYP1A1*2C in Miao, Buyi populations were 79.6%, 20.4% and 81.6%, 18.4%, respectively. Conclusion The distribution of genetic frequency and allele gene frequency of CYP1A1*2CC are without statistically significance in difference between Miao, Buyi nationalities in Guizhou Province China.
下载:
