PAS-Na对染锰大鼠学习记忆及海马神经微丝蛋白表达的影响
Effects of Sodium Para-aminosalicylic Acid on Learning, Memory and Neurofilament Expression in Hippocampus of Manganese-exposed Rats
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摘要:
目的 探讨对氨基水杨酸钠(PAS-Na)对染锰大鼠学习记忆功能及神经微丝蛋白(NF)表达的影响。 方法 将48只雄性SD大鼠随机分为对照组、染锰组、PAS-Na低、高剂量治疗组(L-PAS、H-PAS)四组,染锰组、L-PAS组、H-PAS组大鼠每日腹腔注射(ip)氯化锰(MnCl2& #183;4H2O,15 mg/kg),每周5 d,共3周。然后,L-PAS、H-PAS组大鼠每日分别于背部皮下注射(sc) PAS-Na 100 mg/kg、200 mg/kg,持续5周。采用Morris水迷宫检测大鼠学习记忆能力,免疫组织化学方法检测海马齿状回颗粒细胞下层微丝蛋白(NF)阳性细胞数。 结果 染锰组逃避潜伏期、游泳总路程均比正常对照组长(P < 0.05);H-PAS组逃避潜伏期和游泳总路程比染锰组缩短(P < 0.05)。染锰组NF阳性细胞数比正常对照组减少(P < 0.01),H-PAS组NF阳性细胞数较染锰组增多(P < 0.01),但是仍达不到正常对照组水平(P < 0.01)。 结论 高剂量PAS-Na对锰致学习记忆能力及海马颗粒细胞受损可能有作用。 Abstract:Objective To explore the effects of sodium para-aminosalicylic acid on learning, memory and expression of nurofilament in hippocampus of manganese-exposed rats. Methods Forty-eight male Sprague-Dawley rats were randomly divided into the control, manganese-exposed, L-PAS and H-PAS groups. The rats were intraperitoneally given manganese chloride (MnCl2& #183;4H2O 15 mg/kg)daily for 3 weeks (5 days/week)and treated with PAS-Na at 100 or 200 mg/kg by subcutaneous injection on back in the following 5 weeks (7 days/week). The ability of learning and memory was tested by Morris Water Maze (MWM)and the expression of neurofilament protein in hippocampal subgranular zone of dentate gyrus was examined by immunohistochemical technique. Results The escape latency and swimming distance were obviously prolonged in the manganese-exposed group as compared with that of the control group (P < 0.05). The escape latency and swimming distance of the rats in the H-PAS group were significantly shorter than that in the manganese-exposed group (P < 0.05). NF positive cells in the manganese-exposed group were distinctly less than that in the control group, and the number of NF was evidently incereased in the H-PAS group, but still not recovered to the level of control group (P < 0.01). Conclusion H-PAS may be an antagonist to manganese exposure by showing reduce the adverse effects on learning, memory and neurons in hippocampal subgranular zone of dentate gyrus in the manganese-exposed rats.
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