邹志辉, 杨翠婵, 李伯灵. 葡萄籽油对镉致大鼠肝脏细胞氧化损伤的保护作用[J]. 环境与职业医学, 2011, 28(12): 754-756.
引用本文: 邹志辉, 杨翠婵, 李伯灵. 葡萄籽油对镉致大鼠肝脏细胞氧化损伤的保护作用[J]. 环境与职业医学, 2011, 28(12): 754-756.
ZOU Zhi-hui , YANG Cui-chan , LI Bo-ling . Protective Effects of Grape Seed Oil on Oxidative Damage in Rat Liver Cells Exposed to Cadmium[J]. Journal of Environmental and Occupational Medicine, 2011, 28(12): 754-756.
Citation: ZOU Zhi-hui , YANG Cui-chan , LI Bo-ling . Protective Effects of Grape Seed Oil on Oxidative Damage in Rat Liver Cells Exposed to Cadmium[J]. Journal of Environmental and Occupational Medicine, 2011, 28(12): 754-756.

葡萄籽油对镉致大鼠肝脏细胞氧化损伤的保护作用

Protective Effects of Grape Seed Oil on Oxidative Damage in Rat Liver Cells Exposed to Cadmium

  • 摘要: 目的 探讨葡萄籽油(grape seed oil,GSO)对氯化镉(CdCl2)诱导大鼠肝脏细胞DNA损伤的保护作用。

    方法 将40只SPF级雌雄各半SD大鼠随机分为对照组(腹腔注射生理盐水和灌胃生理盐水)、染镉组(腹腔注射1.0 mg/kg的CdCl2和灌胃生理盐水)、GSO低、中、高剂量干预组(均腹腔注射1.0 mg/kg CdCl2,同时分别灌胃0.66、1.32、2.64 mg/kg GSO),硫代巴比妥酸法检测肝中丙二醛(malondialdehyde,MDA)的含量,采用单细胞凝胶电泳技术(SCGE)检测各组肝脏细胞DNA损伤情况。

    结果 1.0 mg/kg CdCl2可诱导大鼠肝脏细胞合成MDA,使其含量明显增加并加速DNA的氧化损伤;GSO高剂量干预组中MDA含量较染镉组差异具有统计学意义(P<0.05),3个GSO干预组MDA的清除率分别为54.55%、54.55%和100.00%,不同剂量的GSO对MDA清除呈较好的剂量-效应关系。各GSO干预组DNA氧化损伤较染镉组均明显下降(P<0.05)。

    结论 一定剂量葡萄籽油可降低大鼠肝脏中MDA含量和镉致DNA氧化损伤,具有明显的抗脂质过氧化损伤的作用。

     

    Abstract: Objective To explore the protective effect of grape seed oil (GSO) on DNA oxidative damage of liver cells in rats exposed to CdCl2.

    Methods A total of 40 SD rats of SPF grade were randomly divided into 5 groups, which were the control group (intraperitoneal injection and gastric perfusion of normal saline with same volume), the CdCl2 exposure-positive group (intraperitoneal injection of 1.0mg/kg CdCl2 and gastric perfusion of normal saline with the same volume), and the GSO low-, medium-and high-dose intervention groups (intraperitoneal injection of 1.0 mg/kg CdCl2 and gastric perfusion of 0.66 mg/kg, 1.32 mg/kg, 2.64 mg/kg GSO, respectively) with 8 of either gender in each group. Malondialdehyde (MDA) content in liver was measured by thiobarbituric acid (TBA) method. DNA oxidative damage was measured by single cell gel eletrophoresis (SCGE).

    Results The 1.0 mg/kg CdCl2 induced a significant increase in MDA content and DNA oxidative damage. The difference of MDA contents between the GSO high-dose intervention group and the CdCl2 exposure-positive group was significant (P<0.05). The clearances in the GSO low -, medium-and high-dose intervention groups were 54.55%, 54.55% and 100.00%, respectively. The DNA damage significantly decreased in the GSO intervention groups in a dose-dependent manner, compared with the CdCl2 exposure-positive group (P<0.05).

    Conclusion A certain dose of grape seed oil can induce a decrease in MDA content and DNA damage in rat liver after cadmium exposure.

     

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