金纳米粒子共振光散射法测金属硫蛋白
Determination of Metallothioneins by Resonance Light Scattering Method with Gold Nanoparticles
-
摘要:
目的 建立并应用金纳米粒子共振光散射(RLS)法,测定人尿样中金属硫蛋白(MTs)的含量。 方法 在pH 4.56 的B-R缓冲液中,MTs 与金纳米粒子结合形成较大聚集体,导致体系RLS 增强,于545 nm波长处RLS 强度与MTs 的浓度有线性关系,据此建立测定MTs 的新方法。 结果 在最佳实验条件下,在545 nm波长处RLS 强度与MTs在7.98& #215;10-9~1.0& #215;10-7 g/mL 范围内线性关系良好,其线性回归方程为ΔIRLS=16.69ρ-23.09,相关系数r=0.9995,检出限为2.39& #215;10-9 g/mL。 结论 本方法操作简便、快速、仪器设备简单,检出限低,灵敏度高,可用于尿中痕量MTs 的测定。 Abstract:Objective To establish a resonance light scattering(RLS)method for the determination of metallothioneins (MTs)based on the interaction of MTs with gold nanoparticles(GNPs). Methods In B-R buffer solution of pH 4.56, the conjugates of MTs-GNPs were formed by Au-S covalent bond between the mercapto group of cysteine of MTs and GNPs, resulting in the enhancement of RLS intensity of the system owing to their aggregating. RLS intensity linearly correlated with the concentration of MTsat λmax=545 nm, which suggested that a new method for determination of MTs could be established. Results Under the optimum conditions, there was a linear relationship between the RLS intensity and the concentration of MTs in the range of 7.98& #215; 10-9-1.0& #215;10-7 g/mL at λmax545 nm. The linear regression equation was ΔIRLS=16.69ρ-23.09 with a correlation coefficient of 0.9995, and the detection limit of 2.39& #215;10-9 g/mL. Conclusion This method is simple, rapid, sensitive and accurate, and the detection limit is also low. It has been applied for detecting trace MTs in urine.
下载:
