洪丽玲, 丁树娟, 朱江波, 朱玉平, 张天宝. 纳米与常规二氧化钛对A549细胞毒性及DNA损伤的比较[J]. 环境与职业医学, 2011, 28(7): 393-397.
引用本文: 洪丽玲, 丁树娟, 朱江波, 朱玉平, 张天宝. 纳米与常规二氧化钛对A549细胞毒性及DNA损伤的比较[J]. 环境与职业医学, 2011, 28(7): 393-397.
HONG Li-ling , DING Shu-juan , ZHU Jiang-bo , ZHU Yu-ping , ZHANG Tian-bao . Comparative Study of Cytotoxicity and DNA Damage Induced by Nano-and Micro-TiO2 Particles on A549 Cells in Vitro[J]. Journal of Environmental and Occupational Medicine, 2011, 28(7): 393-397.
Citation: HONG Li-ling , DING Shu-juan , ZHU Jiang-bo , ZHU Yu-ping , ZHANG Tian-bao . Comparative Study of Cytotoxicity and DNA Damage Induced by Nano-and Micro-TiO2 Particles on A549 Cells in Vitro[J]. Journal of Environmental and Occupational Medicine, 2011, 28(7): 393-397.

纳米与常规二氧化钛对A549细胞毒性及DNA损伤的比较

Comparative Study of Cytotoxicity and DNA Damage Induced by Nano-and Micro-TiO2 Particles on A549 Cells in Vitro

  • 摘要: 目的 比较纳米二氧化钛(nano-TiO2)和常规TiO2对细胞的毒性及DNA损伤有无差异。

    方法 体外培养人肺腺癌细胞(A549细胞),采用四甲基偶氮唑盐比色法(MTT)和单细胞凝胶电泳技术(彗星实验)比较25、50、100、200μg/mL质量浓度(以下简称"浓度")的nano-TiO2 (5~10 nm)和常规TiO2的细胞毒性与DNA损伤作用。

    结果 nano-TiO2对A549细胞的毒性与常规TiO2的变化趋势相近, nano-TiO2毒性大于常规TiO2, 200μg/mL浓度时作用12 h以上两者细胞存活率的差异有统计学意义(P<0.05)。与对照组相比, nano-TiO2各剂量组尾DNA含量、Olive尾距、彗星尾长均明显增加(P<0.05);常规TiO2在受试剂量下尾DNA含量、Olive尾距、彗星尾长与对照组比较均无明显增加。

    结论 nano-TiO2对A549细胞的毒性高于常规TiO2,且nano-TiO2可诱导DNA损伤,而常规TiO2未观察到DNA损伤作用。表明nano-TiO2与常规TiO2在毒性大小和毒作用性质均有差异。

     

    Abstract: Objective To compare the difference between nano-and micro-TiO2 particles in their cytotoxicity and DNA damage effects on A549 cells.

    Methods A549 human lung adenocarcinoma cells were exposed to different concentrations of these two particles (0, 25, 50, 100 and 200 μg/mL)separately, the methyl thiazolyl tetrazolium (MTT)assay and the single cell gel electrophoresis (comet assay) were applied to measure the cytotoxicity and DNA damage.

    Results It showed that the developed trend of the cytotoxicity induced by nano-TiO2 and micro-TiO2 was similar, but at the same level, nano-TiO2 induced greater damage than the micro-TiO2. For example, after 12 h treatment, nano-TiO2 in a dose of 200 μg/mL inhibited the viability of A549 cells significantly compared with the micro-TiO2 group. In addition, compared to the control group, the tail DNA content, Olive tail moment and comet tail length of the nano-TiO2 group increased significantly (P<0.05); whereas these indices in micro-TiO2 group did not change.

    Conclusion Our data demonstrates that nano-TiO2 particles possess a toxic potential towards A549 cells which may be significantly higher than micro ones; the toxic effects occur earlier in time and lower in dose compared with micro-TiO2, and nano-TiO2 particles can induce DNA damage, but micro-ones do not. It is shown that the nano-and micro-TiO2 particles have some differences in toxicity and toxic properties.

     

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