石墨炉原子吸收光谱法直接测定血中铅

Direct Determination of Lead in Blood by Graphite Furnace Atomic Absorption Spectrometry

摘要: 目的建立稀硝酸稀释血样,石墨炉原子吸收直接测定血铅的方法。

方法采用1.0%硝酸10倍稀释血样,10 μL进样,标准加入法制备标准曲线,直接检测血铅。

结果在实验条件下,血铅浓度在0.0~100.0 μg/L之间存在线性关系(r=0.999 2)。铅标准溶液25.0、50.0、75.0 μg/L分别平行测定6次,其相对标准偏差为1.28%~2.74%。血样中加入铅标准溶液25.0、50.0、75.0μg/L,测得血铅回收率为95.7%~102.0%。

结论该法灵敏度高、正确度好,操作简便快速,适宜血液中铅的测定。

Abstract: Objective To establish a direct blood lead determination methodology by graphite furnace atomic absorption spectrometry using nitric acid treated samples.

Methods Blood samples were pre-treated by 0.1% nitric acid and injected to a graphite furnace atomic absorption spectrometer.

Results The linear range of proposed determination methodology was 0.0-100.0 μg/L (r=0.999 2).The standard solutions containing lead at 25.0,50.0 and 75.0 μg/L were tested six times under the same conditions.The relative standard deviations were 1.28%-2.74% andthe average recoverty-ratios were 95.7%-102.0% for blood samples adding to the same series standard lead solutions.

Conclusion The direct blood lead determination method shows high sensitivity,good linear range and convenience in operation.