王艳艳, 姜红梅, 安玉, 邵小翠, 朴丰源. 三氧化二砷对小鼠大脑组织神经递质代谢酶基因及其受体基因表达谱的影响[J]. 环境与职业医学, 2012, 29(11): 671-673.
引用本文: 王艳艳, 姜红梅, 安玉, 邵小翠, 朴丰源. 三氧化二砷对小鼠大脑组织神经递质代谢酶基因及其受体基因表达谱的影响[J]. 环境与职业医学, 2012, 29(11): 671-673.
WANG Yan-yan , JIANG Hong-mei , AN Yu , SHAO Xiao-cui , PIAO Feng-yuan . Influence of Arsenic Trioxide on Gene Expression Profiles of Metabolic Enzymes and Receptors for Neurotransmitters in Cerebrum of Mice[J]. Journal of Environmental and Occupational Medicine, 2012, 29(11): 671-673.
Citation: WANG Yan-yan , JIANG Hong-mei , AN Yu , SHAO Xiao-cui , PIAO Feng-yuan . Influence of Arsenic Trioxide on Gene Expression Profiles of Metabolic Enzymes and Receptors for Neurotransmitters in Cerebrum of Mice[J]. Journal of Environmental and Occupational Medicine, 2012, 29(11): 671-673.

三氧化二砷对小鼠大脑组织神经递质代谢酶基因及其受体基因表达谱的影响

Influence of Arsenic Trioxide on Gene Expression Profiles of Metabolic Enzymes and Receptors for Neurotransmitters in Cerebrum of Mice

  • 摘要: 目的 研究亚慢性砷暴露对小鼠大脑组织神经递质代谢酶基因及其受体基因表达谱的影响。

    方法 小鼠按体重随机分为3 组,每组2 只:4 mg/L 三氧化二砷(As2O3)染毒组、1 mg/L As2O3 染毒组和生理盐水对照组。染毒组小鼠饮用含As2O3 自来水,对照组饮用0.9%生理盐水,各组小鼠每天消耗水量约20 mL,均连续饮用60 d,之后对其大脑组织进行基因芯片研究。

    结果 与对照组比较,染砷组小鼠大脑组织的基因Th、Dbh、Tph1、Drd3、Drd4、Adra1a、Adra2a、Adra2bAdrb3 表达下调,而基因Htr1f、Htr4、Htr7 表达上调。

    结论 As2O3 可能导致小鼠大脑组织部分神经递质合成和分解代谢酶基因表达下调,且干扰其部分受体基因的表达。

     

    Abstract: Objective To study the effect on gene expression profiles of metabolic enzymes and receptors for neurotransmitters in cerebrum of mice by chronic exposure.

    Methods The rats were randomly divided into three groups:4 mg/L arsenic trioxide (As2O3), 1 mg/L As2O3, and the control groups. After 60 days of exposure to As2O3 through drinking water, expressions of target genes in cerebrum of mice were studied by genechip.

    Results Compared to the control group, the expressions of gene Th, Dbh, Tph1, Drd3, Drd4, Adra1a, Adra2a, Adra2b, and Adrb3 of mouse cerebrum in two treated groups were statistically down-regulated, while the expressions of gene Htr1f, Htr4, and Htr7 were up-regulated.

    Conclusion As2O3 may down-regulate the gene expressions of neurotransmitter synthesis and catabolism enzymes and interfere with the gene expressions of partial neurotransmitter receptors in mouse cerebrum.

     

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