灰霾天气细颗粒物对人支气管上皮细胞凋亡的影响
Effects of Haze Fine Particulate Matter on Apoptosis of Human Bronchial Epithelial Cells
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摘要:
目的 探讨灰霾天气细颗粒物(PM2.5)对人支气管上皮细胞(human bronchial epithelial cells, 16-HBE)凋亡的影响。 方法 分别以 8、16、32、64、128 μg/mL浓度的 PM2.5作用于 16-HBE 24、48、72 h, 检测 PM2.5对 16-HBE细胞存活率及凋亡率的影响。 结果 染毒 24、48、72 h后, 64、128 μg/mL组细胞存活率均明显低于对照组(P < 0.05),细胞凋亡率均明显高于对照组(P < 0.05); 72 h各浓度组细胞存活率均低于 24 h相应处理组(P < 0.05), 当染毒浓度大于8 μg/mL时, 72 h各浓度组细胞总凋亡率与 24 h相比均明显增加(P < 0.05)。 结论 灰霾天气细颗粒物 PM2.5对 16-HBE具有细胞毒性, 能诱导其凋亡, 且具有剂量和时间反应关系。 Abstract:Objective To explore the effect of haze particulate matter PM2.5 on apoptosis of human bronchial epithelial cells (16-HBE). Methods Groups of cultured 16-HBE cells were exposed to 8, 16, 32, 64 and 128μg/mL PM2.5 respectively for 24, 48 and 72 h of incubation to detect the cell survival and apoptosis rates. Results After 24, 48 and 72 h of interference, compared with the negative control group, the cell survival rates of 64 and 128μg/mL groups decreased significantly (P < 0.05), and the cell total apoptosis rates increased significantly (P < 0.05). Compared with the corresponding 24 h groups, the cell survival rates of 72 h groups decreased significantly (P < 0.05), and the apoptosis rates increased significantly except when treated with 8 μg/mL (P < 0.05). Conclusion Haze particulate matter PM2.5 possess cytotoxic effect on 16-HBE to induce apoptosis in a dose-and time-dependent manner.
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