石墨炉原子吸收光谱法测定全血中铍
Determination of Beryllium in Whole Blood with Graphite Furnace Atomic Absorbance Spectrometry
-
摘要:
目的 建立石墨炉原子吸收光谱法测定全血中铍(Be)的方法。 方法 全血样用 5%(v/v)硝酸脱去蛋白,经离心后取上清液进样, 以 0.5%(m/v)硝酸镁 Mg(NO3)2作为基体改进剂, 采用标准工作曲线法测定。 结果 测定方法的线性范围为 0.18~5.00μg/L, 相关系数 (r)为 0.999 6, 相对标准偏差 (relative standard deviation, RSD)为 2.7%~4.2%,检出限为 0.27 μg/L, 加标回收率为 90%~100%。 结论 该方法取样量少, 消解处理简单, 且快速、准确, 适用于全血中铍的测定。 Abstract:Objective To establish the method for determination of beryllium in whole blood with graphite furnace atomic absorbance spectrometry. Methods After the protein in whole blood was removed by nitric acid 5% (v/v) and centrifugal separation, it was determined by standard working curve using Mg (NO3)2 0.5% (m/v) as matrix modifier. Results The linearity range of the method was 0.18-5.00 μg/L, the correlation coefficient (r) was 0.999 6, the relative standard deviation (RSD) was 2.7%-4.2%, the detection limit was 0.27 μg/L, and the recovery was 90%-100%. Conclusion This method is characterized by less amount of samples, simple digestion, better degree of precision and accuracy. It is fit to determine beryllium in whole blood.