内毒素致大鼠肝组织氧化应激作用
Oxidative Stress in Rat Liver Tissues Induced by Endotoxin
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摘要:
目的 探讨内毒素腹腔注射染毒对大鼠肝组织抗氧化酶的影响。 方法 采用腹腔注射技术,以脂多糖(LPS)对Wister大鼠进行染毒,染毒浓度分别为0.01、0.05、0.10、1.00 mg/kg,对肝组织匀浆上清液中的超氧化物歧化酶(SOD)、Cu,Zn-SOD酶、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活性以及谷胱甘肽(GSH)和丙二醛(MDA)含量进行测定。 结果 与对照组相比,染毒浓度分别为0.01、0.05和0.10 mg/kg时,SOD酶活性变化无统计学差异(P>0.05);染毒浓度为1.00 mg/kg时,有明显降低(P<0.01)。与对照组相比,Cu,Zn-SOD酶活性随着染毒浓度的增大,呈现先升后降的趋势,浓度达0.05 mg/kg时,升高具有统计学意义(P<0.01)。与对照组相比,CAT酶活性和MDA含量的变化呈现先降后升的趋势,二者下降的最低点分别为0.05 mg/kg和0.01 mg/kg;GSH含量和GSH-PX活性均呈现先增后减的趋势,染毒浓度为0.05 mg/kg时,二者的增高具有统计学意义(P<0.01)。 结论 低浓度的LPS可致大鼠肝组织产生适应性反应;高浓度的LPS可引起大鼠肝组织抗氧化能力降低,提示高浓度LPS对大鼠肝的毒作用可能与诱发体内产生过量自由基有关。 Abstract:Objective To explore the influences of endotoxin via intraperitoneal injection on several antioxidant enzymes in livers of rats. Methods The rats received injection of lipopolysaccharides (LPS) at different doses of 0.01, 0.05, 0.10, 1.00mg/kg. The activity of superoxide dismutase (SOD), Cu, Zn-SOD enzyme, catalase (CAT) and glutathione peroxidase (GSH-PX), and the content of GSH and malonaldehyde (MDA) in the supernatant of hepatic tissue homogenate were determined. Results Compared with the control group, there was no significant difference of SOD activity in the rats treated with 0.01, 0.05 and 0.10 mg/kg LPS (P>0.05), but it decreased significantly at 1.00 mg/kg (P<0.01); the activity of Cu and Zn-SOD increased first and then decreased, and a significant difference was found in the 0.05 mg/kg group (P<0.01); the activity of CAT and the content of MAD decreased first and then increased, and they declined to the lowest point when treated with 0.05mg/kg and 0.01mg/kg respectively; the activity of GSH-PX and the content of GSH increased first and then decreased, and a significant difference was found in the 0.05 mg/kg group (P<0.01). Conclusion Low dose of LPS can induce adaptive responses in rat liver tissues, while high dose of LPS can lead to decrease of antioxidation in rat liver tissues, indicating that the toxic effects of LPS on rat livers may involve inducing excessive free radicals.
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