依达拉奉对帕金森病小鼠黑质多巴胺能神经元的形态学影响
Effects of Edaravone Administration on Morphological Changes of Dopaminergic Neurons in Substantia Nigra of Parkinson's Mice
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摘要:
目的 探讨依达拉奉对帕金森病(Parkinson's disease,PD)小鼠黑质多巴胺神经元的作用,为依达拉奉临床PD治疗提供实验依据。 方法 将30只健康雄性C57BL/6小鼠随机分为对照组、模型组和治疗组,每组10只。模型组和治疗组给予腹腔注射百草枯(paraquat)和代森锰(maneb)建立PD模型,完成建模后,治疗组给予依达拉奉治疗。观察3组小鼠行为学的改变、并用免疫组织化学和体视学方法对黑质酪氨酸羟化酶阳性神经元进行计数和形态计量分析。 结果 模型组与治疗组比较,自主运动活性明显减少,差异有统计学意义(P<0.05);与治疗组相比,模型组黑质酪氨酸羟化酶阳性神经元数量明显降低,细胞胞体肿胀,平均截面积、平均周长和平均体积明显增大,差异均有统计学意义(P<0.05或P<0.01);胞浆内染色极为浅淡,轴突减少或消失,细胞轮廓不清。 结论 依达拉奉对PD小鼠黑质多巴胺能神经元有明显的神经保护作用。 Abstract:Objective To investigate the protective effect of edaravone on substantia nigra dopaminergic neurons in mice with Parkinson's disease (PD). Methods A total of 30 male C57BL/6 mice were randomly divided into control group, model group and treatment group. Model group and treatment group were intraperitoneally injected with paraquat (PQ) and maneb (Mb) to establish PD model. Treatment group were then administrated with edaravone. Their motor coordination and expression of tyrosine hydroxylase (TH) in the substantia nigra pars compacta (SNc) were detected by immunohistochemistry and stereology. Results Administration of edaravone significantly attenuated the impairment of behavioral performance (P<0.05), increased the number of TH-positive neurons and improved the cell morphology such as cell body, average sectional area, circumference and volume in SNc (P<0.05 or P<0.01). Conclusion The present findings suggest that edaravone has a beneficial influence on nigrostriatal dopaminergic neurons in PD mice.
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