甲基叔丁基醚对加油站职业人群血清淋巴细胞DNA的损伤
Lymphocyte DNA Damage Induced by Methyl Tert-Butyl Ether in Gas Station Attendants
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摘要:
目的 探索汽油添加剂甲基叔丁基醚(methyl tert-butyl ether,MTBE)对加油站职业暴露人群DNA的损伤。 方法 选择华南地区8 家加油站工作人员100 名为调查对象(其中暴露组61 人,对照组39 人),进行健康检查和问卷调查;调查对象各抽取外周静脉血样5 mL,分离血清和淋巴细胞,运用气相色谱-质谱(GC/MS)联用技术测定血清MTBE含量;运用彗星试验(comet assay),分析外周静脉血淋巴细胞DNA 损伤情况;运用Pearson 相关分析探索样本人群血清MTBE 含量与淋巴细胞DNA 损伤的相关性。 结果 接受体检的加油站调查对象未发现身体健康状况不合格者,均符合汽油从业人员的职业卫生要求;暴露组血清MTBE 含量平均值为(6.230& #177;2.369)μg/L,高于对照组(5.164& #177;2.139)μg/L(P<0.05);暴露组Olive 尾矩平均值为(0.060& #177;0.045)μm,也高于对照组(0.039& #177;0.038)μm(P<0.05);Pearson 相关分析显示,暴露组血清MTBE含量与淋巴细胞DNA损伤程度之间呈正相关关系(r=0.859,P<0.05)。 结论 血清MTBE含量与淋巴细胞DNA损伤可能存在相关性。 Abstract:Objective To probe the DNA damages induced by a gasoline additive, methyl tert-butyl ether (MTBE), in occupational exposed population. Methods Physical examination and questionnaire survey were conducted in 100 workers selected from 8 gas stations in South China. A 5 mL peripheral venous blood sample was collected from each worker. Serum and lymphocytes were separated. The content of serum MTBE was detected with gas chromatography-mass spectrometry (GC/MS). Peripheral blood lymphocyte DNA damage was analyzed by comet assay. The relationship between serum MTBE content and lymphocyte DNA damage was explored using Pearson correlation analysis. Results All selected workers met the basic health requirements for gasoline station operators. The mean value of MTBE in the exposure group was (6.230& #177;2.369) μg/L, significantly higher than that in the control group, (5.164& #177;2.139) μg/L (P< 0.05). The mean value of Olive tail moment in the exposure group was (0.060& #177;0.045) μm, significantly higher than that in the control group, (0.039& #177;0.038) μm (P< 0.05). The results of Pearson correlation analysis showed a positive correlation between MTBE content and the degree of DNA damage (r=0.859, P< 0.05). Conclusion There is a correlation between serum MTBE content and lymphocyte DNA damage.
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