混苯接触工人遗传损伤和代谢酶基因多态性的关系
Association between Polymorphisms and Chromosomal Damages of Metabolic Enzyme Genes in Benzene-Mixture-Exposed Workers
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摘要:
目的 探讨混苯接触工人外周血淋巴细胞染色体损伤状况及其与代谢酶基因 CYP2E1和 NQO1多态性的关系。 方法 采用胞质分裂阻滞微核实验评价 461名混苯作业工人和 88名对照组工人的染色体损伤水平,应用聚合酶链反应-限制性内切酶片段长度多态性检测 CYP2E1 Pst和 NQO1609基因多态性。 结果 接触组和对照组淋巴细胞微核率分别为(2.12& #177;1.87)‰和(1.19& #177;1.68)‰,差异有统计学意义(P<0.01),单因素和多因素分析结果均显示,NQO1609野生纯合者(CC)为染色体损伤的易感人群。年龄增长是微核率增加的危险因素,未发现性别、吸烟、饮酒与微核率之间的关系。 结论 双核淋巴细胞微核数可以作为苯接触早期健康损害的指标。混苯中甲苯和二甲苯可能不是导致遗传损伤的主要物质。混苯接触诱导的染色体损伤与 NQO1609位点多态有关。 Abstract:Objective To explore the association between chromosomal damage induced by benzene mixtures and the genetic polymorphisms of metabolic enzymes CYP2E1 and NQO1. Methods Cytokinesis-block micronucleus (CBMN) assay was used to detect chromosomal damage in peripheral lymphocytes of 461 benzene-mixture-exposed workers and 88 controls. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was applied to detect polymorphisms of CYP2E1 Pst and NQO1609. Results The CBMN frequency in the exposed workers was significantly higher than that in the control group(2.12& #177;1.87)‰ vs. (1.19& #177;1.68)‰, P<0.01. The individuals with the NQO1609 CC genotype showed significantly higher CBMN frequencies than the individuals with TT genotype (P<0.05) in either single or multiple factor analysis. Age was an effect modifier for elevated CBMN frequency, while sex, smoking, and alcohol consumption had no relationship with CBMN frequencies. Conclusion Methylbenzene or dimethylbenzene exposure is not a key contributor to genetic damages. Cytokinesisblock micronucleus can be used to detect the early genetic damage of benzene mixture-exposed workers. NQO1609 polymorphisms are associated with chromosomal damage induced by benzene mixtures.
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