孙佩君, 刘建烽, 冯三畏, 肖卫. 二甲基甲酰胺对V79细胞的毒性作用[J]. 环境与职业医学, 2013, 30(4): 268-270.
引用本文: 孙佩君, 刘建烽, 冯三畏, 肖卫. 二甲基甲酰胺对V79细胞的毒性作用[J]. 环境与职业医学, 2013, 30(4): 268-270.
SUN Pei-jun , LIU Jian-feng , FENG Sanwei , XIAO Wei . Toxic Effects of N, N-Dimethylformamide on V79 Cells in Vitro[J]. Journal of Environmental and Occupational Medicine, 2013, 30(4): 268-270.
Citation: SUN Pei-jun , LIU Jian-feng , FENG Sanwei , XIAO Wei . Toxic Effects of N, N-Dimethylformamide on V79 Cells in Vitro[J]. Journal of Environmental and Occupational Medicine, 2013, 30(4): 268-270.

二甲基甲酰胺对V79细胞的毒性作用

Toxic Effects of N, N-Dimethylformamide on V79 Cells in Vitro

  • 摘要: 目的 了解二甲基甲酰胺(DMF)对中国仓鼠肺成纤维细胞(V79 细胞)的细胞毒性及DNA 损伤作用。

    方法 以体外培养V79 细胞为研究对象,采用四氮唑盐比色分析法(MTT 法)和单细胞凝胶电泳技术(SCGE)分别检测5 个浓度(0.5、2.0、8.0、32.0、128.0 mmol/L)DMF 在3 个时间段(6、12、24 h)染毒后对V79 细胞的毒性作用和DNA损伤情况。

    结果 同一时间组V79 细胞存活率随染毒浓度的增加而下降,与阴性对照组比较差异均有统计学意义(P<0.05),存在明显的剂量-效应关系(6 h:b=-0.002,P<0.05;12 h:b=-0.003,P<0.05;24 h:b=-0.003,P<0.05)。各染毒浓度组的彗星拖尾率、尾长、Olive尾距、尾部DNA百分含量,与同一时间阴性对照组比较,差异均有统计学意义(P<0.05)。

    结论 本实验条件下,DMF 能够明显抑制V79 细胞增殖,存在正向的剂量-效应关系,并能引起DNA损伤。

     

    Abstract: Objective To investigate the cytotoxicity and DNA damage in Chinese hamster lung fibroblast cells (V79 cells) induced by N, N-dimethylformamide (DMF) in vitro.

    Methods Tetrazolium (MTT) assay and single cell gel electrophoresis were used to detect the cytotoxicity and DNA damage in V79 cells after exposure to 0.5, 2.0, 8.0, 32.0, and 128.0 mmol/L of DMF for 6 h, 12 h, and 24 h.

    Results DMF significantly reduced the viability of cultured V79 cells with the same exposure time periods (P<0.05) and in a dose-dependent manner (6 h, b=-0.002, P<0.05; 12 h, b=-0.003, P<0.05; 24 h, b=-0.003, P<0.05). There were significant differences in the comet tails rate, tail length, Olive tail moment, and tail DNA percentage between different exposure groups and the control group for the same exposure time periods (P<0.05).

    Conclusion Under current experimental condition, DMF can obviously inhibit the proliferation of V79 cells, showing a positive dose-dependent relationship, and can cause DNA damage.

     

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