章乐怡, 洪程基, 李毅, 郑文力, 马雪莲. 温州市部分公共场所集中空调冷却水军团菌污染状况[J]. 环境与职业医学, 2013, 30(4): 274-277.
引用本文: 章乐怡, 洪程基, 李毅, 郑文力, 马雪莲. 温州市部分公共场所集中空调冷却水军团菌污染状况[J]. 环境与职业医学, 2013, 30(4): 274-277.
ZHANG Le-yi , HONG Cheng-ji , LI Yi , ZHENG Wen-li , MA Xue-lian . Legionella Pollution in Cooling Water of Central Air Conditioning Systems in Public Places of Wenzhou[J]. Journal of Environmental and Occupational Medicine, 2013, 30(4): 274-277.
Citation: ZHANG Le-yi , HONG Cheng-ji , LI Yi , ZHENG Wen-li , MA Xue-lian . Legionella Pollution in Cooling Water of Central Air Conditioning Systems in Public Places of Wenzhou[J]. Journal of Environmental and Occupational Medicine, 2013, 30(4): 274-277.

温州市部分公共场所集中空调冷却水军团菌污染状况

Legionella Pollution in Cooling Water of Central Air Conditioning Systems in Public Places of Wenzhou

  • 摘要: 目的 了解温州市公共场所集中空调冷却水中军团菌污染状况。

    方法 分别于2010 年、2011 年共抽取温州市区30 家公共场所包括医院、宾馆(酒店)、超市(商场)集中空调系统的冷却水126 份。采用分离病原培养法及荧光定量聚合酶链反应(PCR)法进行军团菌检测。

    结果 公共场所冷却塔水的军团菌检出率,2010 年为62.50%,2011 年为33.93%。共分离56 株军团菌菌株,33 株为嗜肺军团菌Lp1~15 型,以Lp6、Lp1 型为优势血清型,其中Lp6 型占19.64%(11/56),Lp1 型占17.86%(10/56),而且主要分布于医院检出的菌株中。荧光定量PCR法检测结果显示,56 株军团菌16S rRNA 均为阳性;且有35 株mip 基因检测阳性,阳性率为62.50%。

    结论 温州市公共场所集中空调冷却水存在军团菌污染,以嗜肺军团菌 LP6、LP1 型为优势菌型。

     

    Abstract: Objective To investigate Legionella pollution in cooling water of central air conditioning system in public places of Wenzhou.

    Methods A total of 126 water samples of cooling towers were collected from 30 public places (hospitals, hotels, and supermarkets) of Wenzhou in 2010 and 2011. Isolated pathogen cultivation and fluorescent quantitative polymerase chain reaction (PCR) were used to detect Legionella organisms in the water samples.

    Results In 2010 and 2011, the positive rates of Legionella were 62.50% and 33.93%, respectively. Of the 56 strains isolated, 33 were identified as Legionella pneumophila Lp1-15, of which 19.64% was Lp6 (11/56) and 17.86% was Lp1 (10/56) and both were predominant serological types and principally derived from hospitals. According to the results of fluorescent quantitative PCR detection, positive 16S rRNA genes were found in 56 Legionella isolates, of which 35 isolates showed mip gene positive, accounting for 62.50%.

    Conclusion Legionella pollution is commonly found in the cooling water of central air conditioning systems in Wenzhou public places, and Lp6 and Lp1 are predominant serological types.

     

/

返回文章
返回