用HOPE-MED8050动式染尘控制系统建立大鼠矽肺模型
Rats Silicosis Model with Dynamic Inhalation Exposure to Silica Dust by HOPE-MED8050 System
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摘要:
目的 采用动式染尘控制系统建立大鼠矽肺模型。 方法 40 只SD大鼠随机分为1 d、3 d、7 d、2 周、4 周、8 周、12 周及对照组,每组5 只;大鼠暴露的染尘浓度为170~190 mg/m3,每天染尘2 h,至各组相应的染尘时间段处死动物(对照组于12 周末处死),称量体重与肺脏重量,计算肺脏器系数,检测肺组织羟脯氨酸(Hyp)含量并对肺组织进行病理组织学观察。 结果 染尘室浓度稳定持久。肺脏器系数随染尘时间延长而逐渐增加;1 d组肺组织轻度充血、水肿;7 d 组血管周围可见较多炎细胞围绕,以单核细胞和淋巴细胞为主;2 周组可见肺泡内有红染物及泡沫细胞;4 周组巨噬细胞呈结节状,肺泡上皮细胞部分脱落,少量纤维组织增生;8 周组纤维性增生更甚,同时可见肺泡II 型上皮细胞腺管样增生;12周组出现纤维性结节,而对照组的大鼠肺部无明显异常。肺组织内Hyp含量随染尘时间延长而增加。 结论 采用动式染尘控制系统可建立大鼠矽肺模型。 Abstract:Objective To establish a rat silicosis model by a dynamic silica dust exposure control system. Methods Forty SD rats were randomly divided into control group, and 1 d, 3 d, 7 d, 2-week, 4-week, 8-week, and 12-week groups, 5 rats for each group. The rats were exposed to silica dust for 2 h a day in a dynamic control system at the concentration of 170-190 mg/m3. After the exposure, the animals were sacrificed according to the assigned group and the control group rats did after 12 weeks. The body weight and lung weight were recorded to calculate lung/body coefficients. Hydroxy proline (Hyp) in the lung tissue was detected and pathological changes were observed. Results The concentration of silica dust in the exposure chamber was maintained stable. The lung coefficient gradually increased with the time of exposure. Mild congestion and edema were observed in the lung tissue in the 1 d group; inflammatory cells around blood vessels were recorded in the 7 d group, most of them were monocytes and lymphocytes. In the 2-week group, red dye and foam cells were found in the alveoli. In the 4-week group, dropped-off alveolar epithelial cells, phagocytes-formed nodules, and a small amount of fibrous tissue hyperplasia were identified. In the 8-week group, more fibrous hyperplasia were observed, plus the gland-like hyperplasia of type II alveolar epithelial cells. Fibrous nodules appeared in the 12-week group, and no abnormality in lung was identified in the control group. The level of Hyp in the lung tissue increased gradually along with the exposure duration to silica dust. Conclusion The rats silicosis model could be well established by the dynamic control system.
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