氟性骨损伤患者血清中骨形成抑制蛋白DKK-1的表达
Expression of Serum DKK-1 Acted as Inhibitor of Bone Formation in Patients with Fluoride Bone Injury
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摘要:
目的 观察氟性骨损伤患者血清dickkopf 相关蛋白1(DKK-1)水平,分析氟性骨损伤的发生与DKK-1 表达的相关性。 方法 采用自行设计的“氟与健康”量表对氟暴露人群进行问卷调查,在调查对象知情同意的前提下,采集静脉血及随机中段尿用于检测血氟、尿氟及血清中DKK-1 蛋白浓度,并对其进行氟斑牙筛检及前臂正位X线检查。根据血氟浓度、氟斑牙及氟性骨损伤程度将调查对象分别分为不同氟负荷组、氟斑牙与否组及不同程度骨损伤组,分析不同组别之间DKK-1 的差异。 结果 血清DKK-1 在低、中、高氟负荷组的浓度分别为(19.60& #177;0.52)、(17.74& #177;0.80)、(14.31& #177;1.20)μg/L,随氟负荷的增高血清DKK-1 浓度依次降低,差异有统计学意义(P<0.05)。无氟斑牙组与氟斑牙组人群血清DKK-1 水平差异无统计学意义(P>0.05)。无、轻、中、重度氟性骨损伤组血清DKK-1 浓度分别为(19.16& #177;0.76)、(15.86& #177;1.35)、(13.27& #177;0.45)、(12.42& #177;0.04)μg/L;骨损伤组血清DKK-1 浓度均低于无骨损伤组,差异有统计学意义(P<0.05)。 结论 氟性骨损伤患者血清DKK-1 表达水平较低,提示高氟暴露可以致机体DKK-1 蛋白表达下调,导致氟性骨损伤的发生。 Abstract:Objective To observe the expression of dickkopf-1 (DKK-1) in serum of fluoride bone injury (FBI) patients and analyze the relationship between fluoride bone injury occurrence and DKK-1 expression levels. Methods General information of fluoride exposed population was collected by a self-designed questionnaire. Venous blood and random midstream urine were sampled from the subjects to detect their serum and urinary fluoride concentrations and serum DKK-1 protein levels followed by informed consent. Dental fluorosis inspection and orthophoric forearm radiography were also performed. Differences in DKK-1 expressions were analyzed among participants grouped by serum fluoride level, dental fluorosis, and skeleton fluorosis diagnostic criteria. Results The concentrations of DKK-1 in the low, middle, and high fluoride burden groups were (9.60& #177; 0.52), (17.74& #177;0.80), and (14.31& #177;1.20) μg/L, respectively, and the difference was statistically significant among the three fluoride burden groups (P<0.05). There was no significant difference in DKK-1 expressions between the dental fluorosis group and the no dental fluorosis group (P>0.05). The levels of DKK-1 in the no, mild, moderate, and heavy FBI groups were (19.16& #177;0.76), (15.86& #177;1.35), (13.27& #177;0.45), and (12.42& #177;0.04) μg/L, respectively, with statistical differences (P<0.05). Conclusion The level of DKK-1 is significantly decreased in patients with FBI. These results also indicate that long-term exposure to high level of fluoride can downregulate DKK-1 expression and might induce occurrence of FBI.
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