上海市88例乙型肝炎患者乙肝病毒基因型与基因突变的关系
Relationship between Genotypes and Mutation of Hepatitis B Virus in 88 Hepatitis B Patients in Shanghai
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摘要:
目的 了解乙型肝炎患者乙肝病毒(hepatitis B virus,HBV)基因型分布及有关位点的基因突变情况。 方法 随机抽取上海市88 例乙肝患者,采用巢式聚合酶链反应检测HBV基因型,扩增HBV Pre-S/S 区和C区序列并测序。 结果 在88 名研究对象中共检出C基因型52 例(59.09%)、B基因型36 例(40.91%)。B基因型的平均年龄为(38.56& #177;12.55)岁,低于C基因型的(44.54& #177;15.72)岁,两组年龄分布差异有统计学意义(P<0.05)。B基因型的乙肝表面E 抗原(HBeAg)阳性率(66.67%)高于C基因型(28.85%),差异有统计学意义(P<0.05)。C基因型C1762、C1764 位点的突变率分别为50.00%和50.77%,分别高于B基因型的27.78%和25.00%,差异均有统计学意义(P<0.05)。 结论 本研究乙型肝炎患者的HBV基因型以C型为主,C基因型C1762、C1764 位点的突变率高于B基因型,应加强HBV基因型和基因突变的监测。 Abstract:Objective To understand the distribution of genotypes and mutation of hepatitis B virus (HBV) in hepatitis B patients. Methods Eighty-eight hepatitis B patients in Shanghai were randomly sampled in 2010. Nested polymerase chain reaction (PCR) was carried out to detect genotypes of HBV in blood samples of the selected patients. The pre-S/S and C gene regions of HBV DNA were amplified and sequenced. Results Among the 88 subjects, 52 patients (59.09%) were infected by HBV genotype C and 36 patients (40.91%) by HBV genotype B. The mean age of HBV genotype B group (38.56& #177;12.55) years old was lower than that of HBV genotype C group (44.54& #177;15.72) years old with significantly statistical difference (P<0.05). The positive rate of hepatitis B e antigen (HBeAg) in the HBV genotype B group (66.67%) was significantly higher than that of the HBV genotype C group (28.85%) (P<0.05). The mutation rates of C1762 and C1764 situs in HBV genotype C group were 50% and 50.77% respectively, statistically higher than those of the genotype B group which were 27.78% and 25% respectively (P<0.05). Conclusion The hepatitis B patients in this study are dominantly infected with genotype C. The mutation rates of C1762 and C1764 situs in patients with genotype C were higher than those with genotype B. Strengthened surveillance on genotype and mutation of HBV is required.
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