氯化钐急性染毒对小鼠肝肾组织影响
Acute Toxic Effect of SmCl3 on Kidney and Liver in Mice
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摘要:
目的 探讨钐对小鼠毒性及对肝、肾脏器系数与抗氧化作用的动态影响。 方法 将昆明小鼠随机分为空白对照组(0.2 mL 生理盐水),低(100 mg/kg)、中(200 mg/kg)、高(400 mg/kg)剂量染毒组,每组30 只。采用一次性腹腔注射氯化钐(SmCl3)染毒,分别于染毒后1、7、14 d 时,计算肝、肾脏器系数、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性并与对照组进行比较。 结果 与对照组相比,钐染毒1 d 后,低、中、高剂量染毒组小鼠肝脏脏器系数均下降(P < 0.05 或P < 0.01),7 d 后差异无统计学意义。高剂量染毒组小鼠肾脏的脏器系数7 d 后高于对照组,差异有统计学意义(P < 0.05)。各染毒组小鼠肝脏、肾脏中MDA含量呈先降低后逐渐恢复的趋势,剂量越低恢复程度越大;肝脏、肾脏中SOD活性增高后相对降低,剂量越大升高幅度越明显。 结论 钐具有诱导SOD和抗氧化功能;肝脏较肾脏对钐染毒SOD活性效应敏感。 Abstract:Objective To investigate the dynamic effects of SmCl3 on organ coefficient and anti-oxidative capacity of kidney and liver in mice. Methods Kunming mice were randomly divided into 4 groups (30 mice in each group): blank control (0.2 mL physiological saline) group, high dose (400 mg/kg) group, moderate dose (200 mg/kg) group, and low dose (100 mg/kg) group. The mice were treated with a single intraperitoneal injection of SmCl3 or physiological saline and neutralized at the 1th, 7th, and 14th days. Organ coefficients of liver and kidney, activities of superoxide dismutase (SOD), and contents of malondialdehyde (MDA) were also measured. Results Compared with the control group, the organ coefficient of liver in each exposure group decreased after 1d (P < 0.05 or P < 0.01), but no difference was observed after 7 d. The organ coefficient of kidney in the high dose group was higher than that of the control group after 7 d (P < 0.05). In the mice administered with SmCl3, the concentrations of MDA in liver and kidney were first decreased and then increased gradually; and the higher recovered MDA concentrations were observed, the lower SmCl3 exposures were administerted. In contrast, the activities of SOD in liver and kidney were first increased and then decreased; and the higher exposure doses were administered, the larger increases were recorded. Conclusion Samarium could induce elevated levels of SOD and anti-oxidative capacity. Liver is more sensitive than kidney to the SOD reaction caused by SmCl3.
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