Objective To measure the levels of a panel of cytokines
interleukin-6(IL-6), IL-17A, IL-17F, IL-21, IL-23 in plasma and the expression of cytochrome P450 1A1(CYP1A1) gene in peripheral leukocytes of residents living around an waste in cinerator.
Methods This present study recruited 42 male residents from a community within 10 km downwind from the waste incinerator (exposure group) and 44 male residents from a community beyond 10 km from the waste incinerator and without known industrial pollution sources (control group). CYP1A1 gene expressions in participants' leukocytes were determined with semi-quantitative reverse transcriptase polymerase chain reaction, and plasma cytokine (IL-6, IL-17A, IL-17F, IL-21, IL-23) levels were measured by Bio-PlexTM Cytokine Panel.
Results The relative CYP1A1 gene expression levels in the exposure group were significantly higher than those in the control group (P<0.05). Also, the plasma levels of IL-17F, IL-21, and IL-23 in the exposure group were significantly higher (P<0.05). After adjusting for age, body mass index, smoking, and alcohol consumption, the transformed levels of IL-17F and IL-23 were associated with exposure (P<0.05) in the multi linear regression analyses.
Conclusion The mRNA expressions of CYP1A1 and the plasma levels of IL-17F and IL-23 in residents living in the vicinity of the waste in cinerator are remarkably higher than those of the residents living further away from the waste incinerator.
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