Objective To evaluate the association between the genetic polylmorphisms of angiotensin II type I receptor(AT1R), angiotensinogen(AGT) and kidney function in adult residents of Pudong New Area of Shanghai.
Methods Residents aged 20-80 years(n=2 026) were randomly selected from Pudong New Area of Shanghai through multistage sampling, excluding those who had ever taken antihypertensive, anti-glycemic, or antilipemic medications, as well as being relatives. Information on demographic characteristics and lifestyle and measurements of height and weight were collected. Plasma levels of creatinine and urinary levels of albumin and creatinine were tested. Urinary albumin-to-creatinine ratio(ACR) and estimated glomerular filtration rate(eGFR) were calculated to evaluate renal function. Single nucleotide polymorphisms at AT1R A1166C and AGT rs699 were genotyped by polymerase chain reaction-ligase detection reaction(PCR-LDR) method.
Results No significant association was observed for genotypes at AT1R A1166C or AGT rs699 with mean age, gender, drinking, or smoking(P> 0.05). For all or any age-stratified participants, no significant association was observed for genotypes at AT1R A1166C or AGT rs699 with any kidney function index such as urinary creatinine, albumin, creatinine, ACR, or eGFR(P> 0.05). In individuals more than 45 years old, the average level of eGFR for AA was lower than that for AC/CC at AT1R A1166C(P< 0.05). The prevalence of albumin or decreased kidney function did not differ by genotypes at AT1R A1166C or AGT rs699 in logistic regression analysis(P> 0.05). The results of generalized linear model analysis showed no interaction between AT1R A1166C and AGT rs699 in relation to the measured phenotypes(P> 0.05). While eGFR was defined as a dependent variable, interaction was found between genotypes at AT1R A1166C and age groups(P=0.039), but eGFR did not differ for genotypes in each age group(P> 0.05).
Conclusion For all the subjects, AT1R A1166C or AGT rs699 genetic polylmorphism is not associated with kidney function, while C mutation at AT1R A1166C associates with higher eGFR in middle-aged and elderly populations.
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